Study on the induction of apoptosis and epithelial-mesenchymal transition by Dioscorea pill combined with gemcitabine in KATO-III Cells
10.3760/cma.j.cn115807-20250109-00025
- VernacularTitle:薯蓣丸联合吉西他滨对KATO-III细胞诱导凋亡及上皮间质转化作用研究
- Author:
Yafei FENG
1
;
Wei GUO
1
;
Jinjiang ZHAO
1
Author Information
1. 长治医学院附属和济医院胃肠外科，长治 046000
- Publication Type:Journal Article
- Keywords:
Dioscorea Pill;
Gemcitabine;
KATO-III cells;
Apoptosis;
Epithelial-Mesenchymal Transition
- From:
Chinese Journal of Endocrine Surgery
2025;19(2):286-290
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism by which the combination of Dioscorea pill and gemcitabine induces apoptosis and epithelial-mesenchymal transition (EMT) in KATO-III cells.Methods:KATO-III cells were used as the experimental model and divided into four groups: control group, Dioscorea pill group, gemcitabine group, and Dioscorea pill combined with gemcitabine group. The cell proliferation was evaluated by CCK-8 assay, apoptosis was detected by flow cytometry, the expression changes of apoptosis-related proteins (such as Bcl-2, Bax, Caspase-3) were analyzed by Western blot, and EMT-related markers (such as E-cadherin, N-cadherin, Vimentin) were analyzed by immunofluorescence and Western blot.Results:Compared with the control group, the Dioscorea pill group, gemcitabine group, and combined treatment group all significantly inhibited the proliferation of KATO-III cells, with the combined treatment group showing the strongest apoptotic induction effect. Flow cytometry results showed that the apoptosis rate in the combined treatment group was significantly higher than that in the single drug groups ( P<0.05) . Western blot results indicated that in the combined treatment group, the expression levels of Bax and Caspase-3 were significantly upregulated, while the expression of Bcl-2 was significantly downregulated. Immunofluorescence and Western blot analysis revealed that combined treatment significantly inhibited the EMT process in KATO-III cells, as evidenced by increased expression of E-cadherin and decreased expression of N-cadherin and Vimentin. Conclusions:Dioscorea pill combined with gemcitabine enhances the anti-tumor effect by promoting apoptosis and inhibiting the EMT process in KATO-III cells. This combination provides a potential new strategy for the clinical treatment of gastric cancer.
