Lycium barbarum polysaccharide inhibits LPS-induced NLRP3 inflammasome mediated inflammatory response in BV2 cells via TLR4/MyD88/NF-κB signaling pathway
10.3969/j.issn.1000-484X.2025.11.017
- VernacularTitle:枸杞多糖通过TLR4/MyD88/NF-κB信号通路抑制LPS诱导的BV2细胞NLRP3炎症小体介导的炎症反应
- Author:
Siwei JIA
1
;
Qin SU
;
Minfang GUO
;
Tao MENG
;
Bingtao MU
;
Jingwen YU
;
Xiaoqin LIU
;
Cungen MA
;
Jiezhong YU
Author Information
1. 山西中医药大学神经生物学研究中心/国家中医药管理局多发性硬化益气活血重点研究室/基于炎性反应的重大疾病创新药物山西省重点实验室,晋中 030606;山西大同大学脑科学研究所/分子细胞免疫学大同市重点实验室,大同 037009
- Publication Type:Journal Article
- Keywords:
Lycium barbarum polysaccharide;
Neuroinflammation;
BV2 microglia;
NLRP3 inflammasome;
Neurodegenerative diseases
- From:
Chinese Journal of Immunology
2025;41(11):2657-2662
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of lycium barbarum polysaccharide(LBP)on lipopolysaccharide(LPS)-induced inflammatory response of NLRP3 inflammasome in BV2 microglial cells.Methods:BV2 microglial cells were routinely cultured.CCK-8 assay was used to detect the effect of different concentrations(0.5,1,1.5,2 g/L)LBP on cell activity.Cells were di-vided into three groups:control group,LPS group and LBP+LPS group.Effect of LBP on LPS-induced cell activity was detected by CCK-8 method;RT-qPCR and immunofluorescence assay were used to detect NLRP3,ASC,Caspase-1,IL-18 and IL-1β expressions.Western blot was used to detect expressions of NLRP3,ASC,Caspase-1,TLR4,MyD88,NF-κB p65,IL-18,IL-1β and TNF-α pro-teins.Results:CCK-8 assay showed that 1 g/L LBP was the most applicable.Compared with control group,cell viability in LPS group was decreased;RT-qPCR,immunofluorescence and Western blot results showed that fluorescence intensity,mRNA and protein expres-sions of NLRP3,ASC,Caspase-1,IL-18 and IL-1β were increased in LPS group.Western blot results showed that TLR4,MyD88,NF-κB p65 and TNF-α protein expressions were increased in LPS group.After LBP treatment,cell viability was increased;expres-sions of NLRP3,ASC,Caspase-1,NF-κB p65,TLR4,MyD88,IL-18,IL-1β and TNF-α were decreased.Conclusion:LBP may in-hibit LPS-induced NLRP3 inflammatory vesicles in BV2 cells via TLR4/MyD88/NF-κB signaling pathway.
