Mechanism of regulating M1/M2 macrophage polarization intervention on intestinal injury of severe acute pancreatitis by Qingjie Huagong decoction
10.3969/j.issn.1000-484X.2025.11.016
- VernacularTitle:清解化攻方调控M1/M2型巨噬细胞极化干预重症急性胰腺炎肠损伤机制研究
- Author:
Hui LI
1
;
Xiaoxia AN
;
Dewen LI
;
Leilei LI
;
Cenyi LIANG
;
Guozhong CHEN
Author Information
1. 广西中医药大学,南宁 530001;广西中医药防治医学分子生物重点实验室,南宁 536008
- Publication Type:Journal Article
- Keywords:
Qingjie Huagong decoction;
Severe acute pancreatitis;
Polarization of macrophages;
Immunity
- From:
Chinese Journal of Immunology
2025;41(11):2651-2656
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Qingjie Huagong decoction on the polarization of intestinal M1/M2 macro-phages in rats with severe acute pancreatitis and the related molecular mechanisms.Methods:Forty-eight SD rats were randomly divid-ed into blank group,model group,Qingjie Huagong decoction low,medium and high dose groups,and Western medicine group.The rat model of severe acute pancreatitis was established according to 5%sodium taurocholate retrograde pancreatic duct.Western medi-cine group was given intraperitoneal injection of ulinastatin injection,and Qingjie Huagong decoction low,medium and high dose groups were given corresponding concentrations of traditional Chinese medicine by intragastric.After 24 h of intervention,pancreatic and ileal tissues were taken for HE staining to observe pathological changes.The levels of serum amylase,TNF-α,IL-6,IL-10 and TGF-β in intestinal tissue homogenate were detected by ELISA.Western blot and Real-time PCR were used to detect phosphatidylino-sitol 3-kinase(PI3K),protein serine-threonine kinase(Akt),inducible nitric oxide synthase(iNOS),leukocyte differentiation anti-gen 86(CD86),and arginase in ileum tissue The relative expression levels of 1(Arg-1),leukocyte differentiation antigen 163(CD163)protein and mRNA.Results:Compared with the model group,the pathological injury of pancreas and small intestine in Qingjie Huagong low,medium and high groups were alleviated after intervention.ELISA method for detecting serum amylase and TNF-α,IL-6 levels significantly decreased(P<0.05),while the levels of IL-10 and TGF-β were increased(P<0.05);expression levels of p-PI3K/PI3K,p-Akt,iNOS,CD86 mRNA and protein were significantly reduced(P<0.05),while the levels of CD163,Arg-1 mRNA and protein were significantly increased(P<0.05),with the highest dose group showing the most significant changes.Conclu-sion:Qingjie Huagong decoction may regulation the PI3K/Akt pathway and interfere with M1 macrophage polarization,inducing M2 macrophage polarization,reducing intestinal inflammatory response,improving intestinal immune barrier,and play its role in anti-in-flammatory-immune regulation.
