Effects of sufentanil on lipopolysaccharide-induced acute lung injury in rats by regulating the cGAS-STING signaling pathway
10.13431/j.cnki.immunol.j.20240100
- VernacularTitle:舒芬太尼调节cGAS-STING信号通路对脂多糖诱导大鼠急性肺损伤的影响
- Author:
Yajing NIU
1
;
Qichao SU
1
;
Ying ZHOU
1
;
Yanping LI
1
;
Yindong XIE
1
Author Information
1. 066000,秦皇岛市第三医院麻醉科
- Publication Type:Journal Article
- Keywords:
Sufentanil;
Cyclic guanosine monophosphate-adenosine monophosphate synthase/stimulator of interferon gene;
Acute lung injury;
Inflammation
- From:
Immunological Journal
2024;40(9):722-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sufentanil(ST)on lipopolysaccharide-induced acute lung injury(ALI)in rats by regulating the cyclic guanosine monophosphate-adenosine monophosphate synthase(cGAS)/stimulator of interferon gene(STING)signaling pathway.Methods Male SD rats were induced by lipopolysaccharide to construct an ALI model.The successfully modeled rats were randomly separated into ALI group,L-ST group,H-ST group,and H-ST+DMXAA group,with 6 rats in each group.Among them,the L-ST group and H-ST group were intraperitoneally injected with 2.5 μg/ml and 5 μg/ml ST after successful modeling immediately.The H-ST+DMXAA group was given 25 mg/kg DMXAA by gavage on the basis of intraperitoneal injection of 5 μg/ml ST.Six healthy and normal temperature rats were randomly selected as the control group,and an equal amount of physiological saline was injected intraperitoneally.The reagent kit was used to detect the SOD enzyme activity and MDA content in serum;ELISA was used to detect the levels of inflammatory factors such as interleukin-10(IL-10),tumor necrosis factor-α(TNF-α),and IL-6 in bronchoalveolar lavage fluid(BALF);cell staining was used to detect the total number of cells and neutrophils in BALF;the wet to dry mass ratio of lung tissue was calculated;HE staining was used to observe pathological changes in lung tissue,while Western blot was applied to detect the expression of cGAS and STING proteins in lung tissue.Results After L-ST and H-ST treatment,the SOD enzyme activity and IL-10 level increased sequentially,the MDA,TNF-α,IL-6,W/D value,total number of cells and neutrophils,cGAS,and STING protein expression decreased sequentially(P<0.05),while lung tissue injury was effectively alleviated.DMXAA reversed the influence of H-ST on the above indexes(P<0.05).Conclusion ST may inhibit the cGAS-STING pathway,reduce inflammation and oxidative stress responses,and thereby alleviates lipopolysaccharide induced ALI in rats.
