Effects of miR-125b on proliferation, invasion and migration of pancreatic cancer cells through targeted regulation of SMYD2 signaling pathway
10.3760/cma.j.cn115807-20240226-00056
- VernacularTitle:miR-125b通过靶向调控SMYD2信号通路对胰腺癌细胞增殖、侵袭和迁移的影响
- Author:
Wenyuan YANG
1
;
Lei MA
;
Xi WANG
;
Xiaolong JIN
Author Information
1. 浙江省台州市中心医院（台州学院附属医院）消化内科，台州 318000
- Publication Type:Journal Article
- Keywords:
miR-125b;
SMYD2;
Pancreatic cancer;
Proliferation;
Invasion;
Migration
- From:
Chinese Journal of Endocrine Surgery
2025;19(3):341-346
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of miR-125b on the proliferation, invasion and migration of pancreatic cancer cells by targeting SMYD2 signaling pathway.Methods:The expression of miRNA-125b in Aspc-1 and BxPC-3 lines of pancreatic cancer cells were detected. miRDB, ENCORI and TargetScan databases were used to predict the potential target genes of miRNA-125b. The downstream target genes of miRNA-125b were identified by qPCR assay and double luciferase reporter gene assay. Western blot analysis was performed to detect SMYD2 protein expression after transfection with miRNA-125b inhibitor. EdU staining, Annexin V-FITC/PI assay and Annexin V-FITC/PI assay were used to detect the effects of miRNA-125b inhibitor transfection and simultaneous transfection of miRNA-125b and SMYD2 inhibitor on cell proliferation, clonogenesis and apoptosis.Results:The expression level of miRNA-125b in pancreatic cancer cell lines was higher than that in normal pancreatic duct cells ( P<0.05). The downstream target gene of miRNA-125b was identified as SMYD2 by qPCR assay and double luciferase reporter gene assay. The expression of SMYD2 protein in miR-125b inhibitor group was higher than that in NC group ( P<0.01). EdU cell proliferation assay showed that the number of miRNA-125b positive cells in inhibitor group was lower than that in NC group and Inhibitor NC group ( P<0.05). The number of clones in miR-125b inhibitor+si-SMYD2 group was more than that in miR-125b inhibitor group ( P<0.01). Annexin V-FITC/PI assay showed that the apoptosis number of cell cells in miR-125b inhibitor+si-SMYD2 group was lower than that in miR-125b inhibitor group ( P<0.01) . Conclusion:miRNA-125b is highly expressed in pancreatic cancer cells, and can directly affect the expression of SMYD2 gene, thereby promoting the proliferation and inhibiting the apoptosis of pancreatic cancer cells.
