Preliminary screening of neutrophil proteomics in diagnosis of pulmonary tuberculosis
10.3969/j.issn.1000-484X.2025.11.021
- VernacularTitle:中性粒细胞蛋白质组学在肺结核诊断中的初步筛选研究
- Author:
Xue LI
1
;
Yuije GAO
1
;
Jiu LIU
1
;
Zikun HUANG
1
;
Junming LI
1
Author Information
1. 南昌大学第一附属医院检验科,南昌 330006
- Publication Type:Journal Article
- Keywords:
Pulmonary tuberculosis;
Neutrophils;
Label-free LC/MS
- From:
Chinese Journal of Immunology
2025;41(11):2682-2688,2694
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the different expression of neutrophil's protein from the patients with active pulmonary tuber-culosis(TB)and healthy controls(HC)from the perspective of proteomics,and to explore the diagnosis of the secreted proteins related to neutrophil activation in serum.Methods:Neutrophil protein in peripheral blood of 15 patients with TB and 15 HC was identified by label free quantitative proteomics,which were further validated in by ELISA and nephelometry.Results:A total of 358 differentially expressed proteins were identified in our study,54 differentially expressed proteins related to neutrophil activation or degranulation were focused,and seven proteins AHSG,AACT,C3,AAT,AAG,HP and A1BG were validated in 40 TB patients and 40 HC,and the results confirmed that the following proteins were highly expressed in the serum of the TB group:AACT,C3,AAT,AAG,HP and A1BG,while AHSG was lowly expressed,and only the validation results of AHSG were consistent with the trend of proteomics results.In addition,we further investigated the expression level of AHSG in the diagnosis of 24 patients with active pulmonary TB,24 patients with lung cancer,24 HC,and 16 patients with latent tuberculosis infection(LTBI),and found the expression of AHSG was down-regu-lated in TB group compared with the other groups.The sensitivity and specificity of AHSG in differentiating TB patients from HC patients,lung cancer patients,LTBI patients and non-TB patients in the test set were 83.33%,79.17%;78.00%,91.67%;75.00%,87.50%;75.00%,85.94%.Conclusion:Proteomic profiles of neutrophils from TB and HC are obtained from a proteomic perspective,and further validation shows that AHSG has a good differential diagnostic ability for HC,lung cancer and LTBI.
