Mechanism of Helicobacter pylori-mediated neutrophil apoptosis disorder

VernacularTitle:幽门螺杆菌介导的中性粒细胞凋亡障碍机制研究
Author: Hongmei BI ¹ ; Chang XU ; Xiaolin SHI ; Dan LI ; Peng LIU
Author Information

1. 威海市立医院,威海 264200
Publication Type:Journal Article
Keywords: Helicobacter pylori; Neutrophils; Apoptosis
From: Chinese Journal of Immunology 2025;41(11):2602-2606
CountryChina
Language:Chinese
Abstract: Objective:To investigate molecular mechanism of Helicobacter pylori(H.pylori)-mediated neutrophil apoptosis disorder.Methods:Neutrophils extracted from peripheral blood of healthy adults were used as research subjects,and H.pylori stan-dard strain NCTC11637 was added to culture system to construct a model of in vitro infection,which was divided into control group(with PBS or medium treatment),H.pylori group(H.pylori co-cultivated with neutrophils at ratio of 10∶1).After a certain time of in-fection,apoptosis-related molecules of neutrophils were detected by flow cytometry,ELISA and Western blot,respectively.Results:Cytokines IL-1β,IL-6 and TNF-α were secreted significantly higher in H.pylori group than control group,apoptosis rate was signifi-cantly lower than control group,apoptosis-related proteins Caspase-3 and Caspase-8 were higher than control group,but their activa-tion level were lower than control group.Conclusion:H.pylori infection may prolong neutrophil survival by inhibiting apoptotic pro-cess of neutrophils through inhibiting activation of Caspase-3/8.