Effect of TNFAIP8 on immune tolerance in kidney transplantation by regulating Treg cells
10.3969/j.issn.1000-484X.2025.11.006
- VernacularTitle:TNFAIP8调节Treg细胞对肾移植中免疫耐受的影响
- Author:
Zhenpu WANG
1
;
Jun FANG
1
Author Information
1. 河南中医药大学第五临床医学院塈郑州人民医院肾移植科,郑州 450003
- Publication Type:Journal Article
- Keywords:
Kidney transplantation;
TNFAIP8;
Treg;
Immune tolerance
- From:
Chinese Journal of Immunology
2025;41(11):2591-2595
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of tumor necrosis factor-α-inducible protein-8(TNFAIP8)regulating CD4+CD25+FOXP3+Treg cells involved in immune tolerance of kidney transplantation model.Methods:Wistar rats were used as recipients of kidney transplantation and divided into control,model,model+TNFAIP8,model+TNFAIP8+CD25 groups,with 12 rats in each group.SD rats were used as kidney donors,and the left kidney was transplanted into Wistar rats.Lentivirus-TNFAIP8(300 μg/kg)was injected into the tail vein to overexpress TNFAIP8.CD25 antibody at 10 mg/kg was injected into the tail vein to inhibit the differen-tiation of Treg.Renal tissue injury,renal function,Treg cell ratio,protein levels of TNFAIP8 and FOXP3 were compared.Results:Compared with control group,BUN[(23.85±20.68)mmol/L]and Scr[(112.49±10.02)μmol/L]in model group were increased,while proportion of Treg cells[(3.01±0.32)%],protein levels of TNFAIP8 and FOXP3 were decreased(P<0.05).BUN[(11.74±11.04)mmol/L]and Scr[(65.56±6.15)μmol/L]in model+TNFAIP8 group were lower than those in model group,Treg level[(6.74±0.68)%],TNFAIP8 and FOXP3 protein levels were higher than those in model group(P<0.05).BUN[(20.26±2.19)mmol/L]and Scr[(100.82±8.27)μmol/L]in model+TNFAIP8+CD25 group were higher than those in model+TNFAIP8 group,while the levels of Treg[(3.34±0.36)%]and FOXP3 protein were lower than those in model+TNFAIP8 group(P<0.05).Conclusion:TNFAIP8 involved in immune tolerance in kidney transplantation by regulating CD4+CD25+FOXP3+Treg cells.
