Repair effect of oleuropein on OGD/R-induced neuronal damage by regulating Hippo-YAP signaling pathway
10.3969/j.issn.1000-484X.2025.11.009
- VernacularTitle:橄榄苦苷调节Hippo-YAP信号通路对OGD/R诱导的神经元损伤的修复作用
- Author:
Yanling DENG
1
;
Zhigang WU
1
;
Qian XIAO
1
;
Ke ZHANG
1
Author Information
1. 海南西部中心医院康复医学科,儋州 571700
- Publication Type:Journal Article
- Keywords:
Oleuropein;
Hippo-Yes associated protein signaling pathway;
Oxygen glucose deprivation/reperfusion;
Neuron damage
- From:
Chinese Journal of Immunology
2025;41(11):2607-2612,2618
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of oleuropein(OE)on oxygen glucose deprivation/reperfusion(OGD/R)in-duced neuronal damage and Hippo-Yes associated protein(YAP)signaling pathway.Methods:Neurons were grouped into Control group,OGD/R group,OE-L group,OE-M group,OE-H group and OE-H+YAP inhibitor(VP)group;detection of cell activity;cell proliferation and apoptosis were detected in each group;the levels of inflammatory factors and oxidative stress were detected;the ex-pression levels of Cyclin D1,cell cycle negative regulator(P21),B cell lymphoma 2(Bcl-2),Bcl-2 associated X protein(Bax),YAP and transcription coactivator with PDZ-binding motif(TAZ)were detected.Results:In OGD/R group,deep staining of neuronal cell nuclei,EdU positive rate,Cyclin D1,Bcl-2,YAP and TAZ expressions were decreased,P21,TNF-α,IL-6,MDA,SOD,apop-tosis rate and Bax expression were increased(P<0.05).The cell nucleus was gradually restored to normal by OE,the blue staining was gradually lightest,the EdU positive rate,Cyclin D1,Bcl-2,YAP and TAZ levels were increased,and the expressions of P21,TNF-α,IL-6,MDA,SOD,apoptosis rate and Bax were decreased(P<0.05);VP reverse protective effect of OE on OGD/R-induced neuronal injury.Conclusion:OE can improve OGD/R-induced neuronal damage by activating Hippo/YAP signaling pathway.
