Ureaplasma spp.LAMPs inhibits ferroptosis by positively regulation of macrophage M1 polarization
10.3969/j.issn.1000-484X.2025.11.003
- VernacularTitle:解脲脲原体LAMPs通过正向调控巨噬细胞M1极化抑制铁死亡机制研究
- Author:
Jinglei BI
1
;
Maojin TIAN
;
Peiqing ZHAO
;
Rongkui ZHANG
Author Information
1. 淄博市妇幼保健院生殖中心,淄博 255029
- Publication Type:Journal Article
- Keywords:
Ureaplasma spp.LAMPs;
Macrophages M1 polarization;
Ferroptosis;
NF-κB
- From:
Chinese Journal of Immunology
2025;41(11):2573-2577
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Ureaplasma spp.lipid-associated membrane proteins(LAMPs)on macro-phage M1 polarization,and clarify how LAMPs resist to ferroptosis by regulating macrophage M1 polarization.The overall goal is to contribute to a better understanding of pathogenesis of non-gonococcal urethritis caused by Ureaplasma spp.Methods:ELISA,qPCR and flow cytometry were used to analyze the effect of LAMPs on M1 polarization of macrophages;Western blot to detect how LAMPs stimulates NF-κB pathway during M1 polarization of macrophages;gene interference technology was used to elucidate how LAMPs affects macrophage M1 polarization through regulating NF-κB pathway;CCK8 and Western blot were used to find out how LAMPs affects macrophage ferroptosis by regulating NF-κB pathway.Results:ELISA,qPCR and flow cytometry results showed that LAMPs promoted macrophage polarization to M1;Western blot results showed that LAMPs increased levels of p-p65 in macrophages;interfering with p65 expression could inhibit M1 polarization process of macrophages that mediated by LAMPs,and inhibition of macrophage ferroptosis that mediated by LAMPs could be significantly reversed.Conclusion:LAMPs inhibits ferroptosis by accelerating macrophage M1 po-larization,which is one of the important pathogenic factors of non-gonococcal urethritis caused by Ureaplasma spp.
