Effects of oleuropein on the migration, invasion, and chemotherapy sensitivity of cervical cancer cells by regulating the PI3K/AKT signaling pathway
10.3760/cma.j.cn115807-20240614-00203
- VernacularTitle:橄榄苦苷调节PI3K/AKT信号通路对宫颈癌细胞迁移、侵袭及化疗敏感性的影响
- Author:
Lina XU
1
;
Chunli WEN
;
Donglin GUO
;
Huiyan LIU
;
Suqin WANG
Author Information
1. 山西省人民医院妇产科，太原 030012
- Publication Type:Journal Article
- Keywords:
Cervical cancer;
Oleuropein;
Phosphatidylinositol 3 kinase-protein kinase B signaling pathway;
Migration;
Invasion;
Chemotherapy sensitivity
- From:
Chinese Journal of Endocrine Surgery
2024;18(6):892-897
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of oleuropein (OLE) on the migration, invasion, and chemotherapy sensitivity of cervical cancer cells by regulating the phosphatidylinositol 3 kinase-protein kinase B (PI3K-AKT) signaling pathway.Methods:SiHa cells were treated with 0-80 μ mol/L OLEs to detect cell survival rate and screen for the optimal drug concentration. SiHa cells were randomly separated into SiHa group, OLE low concentration (OLE-L) group, OLE medium concentration (OLE-M) group, OLE high concentration (OLE-H) group, and OLE-H+PI3K activator 740Y-P (OLE-H+740Y-P) group. CCK-8 method was applied to detect the proliferation activity of cells in each group. Transwell experiment was applied to detect the migration and invasion abilities of cells in each group. Flow cytometry was applied to detect the apoptosis rate of cells in each group. Western blot was used to detect the expression levels of PI3K-AKT signaling pathway related proteins, epithelial mesenchymal transition (EMT), and resistance related proteins such as epithelial cadherin, vimentin, N-cadherin, and P-glycoprotein (P-gp) of cells in each group.Results:OLEs with concentrations of 5, 10, and 20 μmol/L were selected for subsequent experiments. Compared with the SiHa group, the survival rate, numbers of clone formation, migration and invasion of cells, and the expression levels of p-PI3K/PI3K, p-AKT/AKT, Vimentin, N-cadherin and P-gp in the OLE-L, OLE-M, and OLE-H groups gradually decreased ( P<0.05), while the apoptosis rate and E-cadherin expression level increased ( P<0.05). Compared to that before cisplatin treatment, the apoptosis rate of SiHa cells increased after cisplatin treatment ( P<0.05). The addition of PI3K activator 740Y-P on the basis of high concentration OLE reversed the trend of changes in the above indicators ( P<0.05) . Conclusions:OLE can inhibit the migration and invasion of cervical cancer cells, and improve their chemotherapy sensitivity. Its mechanism of action may be related to the inhibition of the PI3K-AKT signaling pathway.
