Study on the effect of protocatechuic acid on the proliferation, migration and apoptosis of ovarian cancer cells and its mechanism
10.3760/cma.j.cn115807-20231010-00099
- VernacularTitle:原儿茶酸对卵巢癌细胞增殖迁移和凋亡的影响和机制研究
- Author:
Chenchen CHEN
1
;
Haizhen JIANG
1
;
Jin CHEN
1
;
Yunfei WANG
1
Author Information
1. 济宁医学院附属医院妇科，济宁 272029
- Publication Type:Journal Article
- Keywords:
Protocatechuic acid;
Ovarian cancer cells;
Proliferation;
Apoptosis
- From:
Chinese Journal of Endocrine Surgery
2025;19(1):80-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the inhibitory effect of protocatechuic acid (PCA) on the growth of ovarian cancer cell line A2780 and its mechanism.Methods:A2780 cells were cultured in vitro and randomly divided into a control group and protocatechuic acid-treated groups with concentrations of 5 μM and 10 μM. Cell proliferation was detected using the MTT assay; Cell migration ability was assessed through wound healing experiments; Cell cycle and apoptosis were analyzed using flow cytometry; Protein expression of apoptosis-related proteins was examined using Western blot.Results:Compared with the control group, the absorbance of the protocatechuic acid groups (5 μM, 10 μM) decreased after 48 hours, and protocatechuic acid significantly inhibited cell proliferation ( P<0.05) . In the 5 μM protocatechuic acid treatment group, the proportion of cells in the G2/M phase increased to 25.26%, and in the 10 μM treatment group, the proportion of cells in the G2/M phase increased to 24.99%. Protocatechuic acid groups could arrest the cell cycle of the human ovarian cancer cell line A2780 at the G2/M phase. Protocatechuic acid groups could also promote the increase of early and late apoptosis rates of A2780 cells, leading to the upregulation of the expression levels of related apoptotic proteins CASPASE-3 and Bax, while the expression level of the anti-apoptotic protein Bcl2 was downregulated. Conclusions:Protocatechuic acid can inhibit proliferation and migration of A2780 ovarian cancer cells, arrest the cell cycle of A2780 cells, and promote apoptosis. The mechanism of action may be related to the upregulation of apoptosis-related proteins BAX and caspase3.
