Expression of transcription factor ZBTB4 in papillary thyroid carcinoma and its relationship with clinicopathological features
10.3760/cma.j.cn115807-20240517-00171
- VernacularTitle:转录因子ZBTB4在甲状腺乳头状癌中的表达及其与肿瘤临床病理特征的关系
- Author:
Lin JIANG
1
;
Yun XI
;
Wangang GONG
;
Jinbiao SHANG
Author Information
1. 浙江省肿瘤医院甲状腺外科，杭州 310022
- Publication Type:Journal Article
- Keywords:
Transcription factor;
ZBTB4;
Papillary thyroid carcinoma;
Hashimoto’s thyroiditis
- From:
Chinese Journal of Endocrine Surgery
2025;19(1):63-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of transcription factor Zinc finger and BTB domain-containing 4 (ZBTB4) in papillary thyroid carcinoma and its relationship with the clinicopathological features of the tumor.Methods:Immunohistochemistry (IHC) was used to assess ZBTB4 protein of cancerous tissues and paired-normal tissues extracted from surgical samples of 60 patients with papillary thyroid carcinoma (30 classical and 30 with Hashimoto’s thyroiditis) . Fresh tissue samples were collected from above patients, and the expression of ZBTB4 gene mRNA and ZBTB4 protein in cancerous tissues was detected by qRT-PCR and Western blot methods. SPSS statistical software was used to analyze the relationship between the expression level of this gene in tumor tissues, clinicopathological features and tumor prognosis.Results:Immunohistochemical results showed that the expression of ZBTB4 protein in papillary thyroid carcinoma tissues was significantly higher than that in normal thyroid tissues (25.0% vs. 3.3%, P=0.001) . The results of qRT-PCR showed that the expression of ZBTB4 gene mRNA in tumor tissues was significantly higher than that in normal thyroid tissues ( P<0.001) . There was no significant difference in the expression level of ZBTB4 between patients with classical papillary carcinoma and those with Hashimoto’s thyroiditis. Multivariate analysis showed that the patients with higher expression of ZBTB4 had relatively smaller tumor diameter ( t=2.31, P=0.025) and less lateral cervical lymph node metastasis ( t=4.13, P=0.042) . Conclusions:Patients with higher ZBTB4 expression level have better outcome, as reflected in smaller tumor diameter and lower rates of lateral cervical lymph node metastasis. The molecular mechanism of ZBTB4 gene in papillary thyroid carcinoma needs further study.
