Influence mechanism of nitrate on the determination of calcium content in pitavastatin calcium by flame atomic absorption spectrometry
10.19778/j.chp.2025.05.014
- VernacularTitle:火焰原子吸收分光光度法测定匹伐他汀钙中钙含量时硝酸根的影响机制研究
- Author:
Zhijun ZHANG
1
;
Shudong ZHANG
1
;
Lin WANG
1
;
Zhe ZHANG
1
;
Qin HU
1
Author Information
1. 北京市药品检验研究院,国家药品监督管理局仿制药研究与评价重点实验室,中药成分分析与生物评价北京市重点实验室,北京 102206
- Publication Type:Journal Article
- Keywords:
flame atomic absorption spectrometry;
pitavastatin calcium;
calcium;
nitric acid;
jamming mechanism
- From:
Drug Standards of China
2025;26(5):538-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of the influence of nitrate on the determination of calcium content in pitavastatin calcium by flame atomic absorption spectrometry.Methods:By comparing the absorbance of calci-um standard test solutions using hydrochloric acid and nitric acid as solution media,and measuring the changes in calcium absorbance in calcium ion solutions prepared by adding different concentrations of nitric acid using flame atomic absorption spectrophotometry,the relationship between calcium atomization efficiency and calcium ion con-centration,nitrate concentration,and their concentration ratios was explored.Results:Nitrate had a significant effect on reducing the absorbance value of the calcium standard test solution;The degree of interference varied with the concentration of the element calcium in the sample,and was related to the ratio of the concentration of the ele-ment calcium to the concentration of the interfering substance nitrate.Moreover,the interference of nitrate on calci-um affected the slope of the working curve.Conclusion:The interference mechanism of nitrate in the determination of calcium by flame atomic absorption spectrophotometry is due to the decomposition of calcium nitrate formed by ni-trate and calcium into difficult to melt,evaporate,and effectively dissociate calcium oxide at high temperatures,which reduces the atomization efficiency of calcium atoms and results in low absorbance values in the measurement.It is not advisable to select calcium standard stock solutions containing nitric acid when determining calcium containing samples such as pitavastatin calcium,and it is also necessary to be vigilant against the interference of nitrate ions.
