Diosgenin glucoside promotes apoptosis of colorectal cancer cells by de-creasing expression of SCD1
10.3969/j.issn.1000-4718.2025.01.012
- VernacularTitle:延龄草苷通过降低SCD1表达促进结直肠癌细胞凋亡
- Author:
Xing WAN
1
;
Fang ZHANG
;
Feng LI
;
Zhigui LI
;
Liming ZHOU
Author Information
1. 湖北民族大学医学部药理教研室,湖北 恩施 445000;四川大学基础医学与法医学院药理教研室,四川 成都 610044
- Publication Type:Journal Article
- Keywords:
diosgenin glucoside;
colorectal cancer;
stearoyl-CoA desaturase 1;
apoptosis
- From:
Chinese Journal of Pathophysiology
2025;41(1):97-103
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the clinical significance of stearoyl-CoA desaturase 1(SCD1)in colorectal can-cer and to evaluate the effects and mechanisms of diosgenin glucoside on the proliferation of colorectal cancer cells.METHODS:The expression of SCD1 in colorectal cancer tissues was assessed using qPCR and Western blot,and its cor-relation with clinical features was analyzed.The impact of diosgenin glucoside on the viability of SW620 and HCT116 cells was measured using the CCK-8 assay,and the proliferation was further investigated through cell cloning experiments.Ad-ditionally,the effects of diosgenin glucoside on apoptosis-related genes and SCD1 were evaluated by qPCR and Western blot.Molecular docking was employed to analyze the binding site and binding energy between diosgenin glucoside and SCD1.RESULTS:(1)Both SCD1 mRNA and protein were highly expressed in colorectal cancer tissues,with an 88%positive rate.And SCD1 mRNA expression correlated with cancer type and clinical stage.(2)Diosgenin glucoside de-creased the viability and cloning ability of SW620 and HCT116 cells in a dose-dependent manner.(3)Diosgenin gluco-side upregulated the mRNA and protein expression of pro-apoptotic proteins caspase-3 and Bax in a dose-dependent man-ner,while it downregulated the mRNA and protein expression of the anti-apoptotic protein Bcl-2.(4)Diosgenin glucoside reduced the mRNA and protein expression of SCD1 in a dose-dependent manner and bound to SCD1 via hydrogen and con-jugated bonds with a binding energy of-7.7 kcal/mol.CONCLUSION:(1)SCD1 is closely associated with the type and clinical stage of colorectal cancer.(2)The reduction in viability and proliferation of colorectal cancer cells by diosgenin glucoside may be attributed to its binding to SCD1,which lowers its expression and promotes apoptosis.
