Comparison of therapeutic efficacy of ferroptosis inhibitor liproxstatin-1 and antioxidant N-acetylcysteine in bleomycin-induced pulmonary fibrosis
10.3969/j.issn.1000-4718.2025.01.004
- VernacularTitle:铁死亡抑制剂liproxstatin-1和抗氧化剂N-乙酰半胱氨酸在博来霉素诱导的肺纤维化中的疗效对比
- Author:
Fang CHEN
1
;
Liang YUAN
1
;
Ge WANG
1
;
Shiting GHEN
1
;
Zeguang ZHENG
1
Author Information
1. 广州医科大学附属第一医院医院呼吸内科,广东 广州 81873408
- Publication Type:Journal Article
- Keywords:
liproxstatin-1;
N-acetylcysteine;
bleomycin;
pulmonary fibrosis;
ferroptosis
- From:
Chinese Journal of Pathophysiology
2025;41(1):28-35
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To compare the therapeutic effects of liproxstatin-1(LIP-1)and N-acetylcysteine(NAC)in bleomycin(BLM)-induced pulmonary fibrosis.METHODS:The mice were randomly divided into the control,model(BLM),BLM+NAC,BLM+LIP-1,NAC and LIP-1 groups.The BLM+NAC and BLM+LIP-1 groups were treated with NAC by intratracheal drip and LIP-1 by intraperitoneal injection,respectively,1 day before BLM tracheal instillation.The other groups received intraperitoneal injection of the same volume of co-solvent and intragastric instillation of saline.Fourteen days after a BLM challenge,the degree of lung fibrosis and the expression levels of alveolar epithelial cell mark-ers and ferroptosis-related molecules were assessed in each group.RESULTS:LIP-1 treatment more significantly im-proved the BLM-induced decrease in body weight(P＜0.01)and survival rate in mice compared with NAC.LIP-1 more significantly reduced the severity of pulmonary fibrosis and improved collagen deposition compared with NAC.LIP-1 also more significantly alleviated alveolar structural disruption,and more significantly inhibited the decrease in the alveolar epi-thelial cell markers podoplanin and surfactant protein C,as well as epithelial-mesenchymal transition,compared with NAC.LIP-1 was a more potent inhibitor of the BLM-induced increase in ferroptosis and its related molecule Heme oxygen-ase-1 than NAC.CONCLUSION:LIP-1 treatment is more effective than NAC in alleviating pulmonary fibrosis.Mecha-nistically,this finding may be related to the ability of LIP-1 to inhibit ferroptosis in alveolar epithelial cells.This study pro-vides new insights into the treatment of pulmonary fibrosis and lays the foundation for the clinical application of LIP-1.
