Isolation,identification and biological characterization of influenza D viruses in Jilin region
10.16303/j.cnki.1005-4545.2025.11.14
- VernacularTitle:D型流感病毒吉林株的分离鉴定及生物学特性分析
- Author:
Hongjin LI
1
;
Hailin JIN
;
Xinxin LIU
;
Weiwen YAN
;
Xianwen LIN
;
Yue YIN
;
Bing GAO
;
Xianyuan KONG
;
Che SONG
;
Guang WANG
;
Renfu YIN
Author Information
1. 吉林大学动物医学学院人畜共患传染病重症诊治全国重点实验室,吉林长春 130062;长春科技学院生命科
- Publication Type:Journal Article
- Keywords:
influenza D virus;
virus isolation and identification;
phylogenetic analysis
- From:
Chinese Journal of Veterinary Science
2025;45(11):2405-2410
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the prevalence of influenza D virus(IDV)in cattle and swine populations in Jilin Province,China,277 nasopharyngeal swabs were collected from livestock exhibiting influ-enza-like symptoms for IDV detection.Virus isolation was performed using swine testicular(ST)cells for PCR-positive samples,followed by comprehensive analyses including whole-genome se-quencing,phylogenetic analysis,electron microscopic observation of viral morphology,and glycosy-lation site prediction.Two IDV strains were successfully isolated from bovine samples,designated as D/bovine/China/JL22/2024(JL22)and D/bovine/China/JL34/2024(JL34).These strains were demonstrated to have specific hemagglutination activity against turkey red blood cells,while no he-magglutination to chicken,rabbit,or guinea pig erythrocytes.Virus-inoculated ST cells exhibited distinct cytopathic effects(CPE)within 48 h,with a hemagglutination titer of 4 log2 in the culture supernatant.Phylogenetic analysis of the hemagglutinin-esterase-fusion(HEF)gene indicated that these strains were most closely related to the Japanese isolate D/Yamagata2019,belonging to the YAMA2019 lineage.Genomic sequence analysis showed the absence of genetic reassortment in these isolates.In this study,two IDV strains were successfully isolated and characterized,which provides preliminary insights into their genomic sequences and biological properties.The findings confirm the presence of IDV in bovine populations in Jilin Province and provide the fundamental data for future epidemiological surveillance and control strategies of IDV.
