Regulatory role of miRNA in the interaction between Pseudopleuronectes yoko-hamae and Edwardsiella tarda
10.16303/j.cnki.1005-4545.2025.11.10
- VernacularTitle:miRNA在黄盖鲽与迟缓爱德华菌互作中的调控作用
- Author:
Yile CHAI
1
;
Huijie WANG
1
;
Jian HAN
1
;
Zhizhi GU
1
;
Wei WANG
1
;
Shengnan CAO
1
Author Information
1. 大连海洋大学 水产与生命学院辽宁省北方鱼类应用生物学与增养殖重点实验室,辽宁大连 116023
- Publication Type:Journal Article
- Keywords:
Edwardsiella tarda;
Pseudopleuronectes yokohamae;
histopathology;
miRNA
- From:
Chinese Journal of Veterinary Science
2025;45(11):2372-2379
- CountryChina
- Language:Chinese
-
Abstract:
Pseudopleuronectes yokohamae is an important marine economic fish species in northern China and is vulnerable to various pathogens during the breeding process.To investigate the viru-lence of Edwardsiella tarda on Pseudopleuronectes yokohamae and elucidate the role of miRNAs in their interaction,we conducted pathological analyses on various tissues of Pseudopleuronectes yokohamae larvae pre-and post-infection with Edwardsiella tarda.Subsequently,Illumina high-throughput sequencing was employed to identify and functionally characterize differentially ex-pressed miRNAs in liver and kidney tissues.The results demonstrated that infection with Ed-wardsiella tarda led to ulceration,hemorrhaging,significant hepatomegaly,nephromegaly,and as-cites in the abdominal cavity.Histopathological examination revealed swollen hepatocytes with vac-uolization,minor blood cell infiltration,and enlarged renal corpuscles with signs of cellular lysis.miRNA sequencing analysis indicated that 24 miRNAs were significantly upregulated and 45 miR-NAs were significantly downregulated in the liver,while in the kidney,6 miRNAs were upregulat-ed and 58 were downregulated following infection.Functional enrichment analysis revealed signifi-cant enrichment of differentially expressed miRNAs in Toll and IMD signaling pathways,HIF-1 signaling pathway,Rap1 signaling pathway,MAPK signaling pathway,and Apelin signaling path-way.These findings suggest the involvement of these miRNAs in host metabolism,pathogen rec-ognition,and immune response in the interaction between Pseudopleuronectes yokohamae and Ed-wardsiella tarda.This research lays a foundation for elucidating the mechanisms underlying bacte-rial disease susceptibility and resistance in Pseudopleuronectes yokohamae.
