The application progress of nanomaterials combined with CRISPR/Cas system in the detection of pathogenic microorganisms
10.16303/j.cnki.1005-4545.2025.11.32
- VernacularTitle:纳米材料结合CRISPR/Cas系统在病原微生物检测中的应用进展
- Author:
Qiuting XIONG
1
;
Zhihao YAN
;
Xuefeng CAO
;
Rendong FANG
;
Mingyuan LIU
;
Xiaoxiang HU
Author Information
1. 西南大学动物医学院,重庆北碚 400715
- Publication Type:Journal Article
- Keywords:
nanomaterials;
CRISPR/Cas system;
pathogenic microorganisms;
detection
- From:
Chinese Journal of Veterinary Science
2025;45(11):2569-2578
- CountryChina
- Language:Chinese
-
Abstract:
Pathogenic microorganisms are direct causative agents of zoonotic infectious diseases,po-sing severe threats to the livestock industry by inducing massive animal mortality,economic losses in livestock products,and significant risks to human health.The CRISPR/Cas system has been widely adopted in nucleic acid detection of pathogenic microorganisms due to its unique trans-cleavage activity.By leveraging the superior optical properties of nanomaterials,researchers have integrated them with CRISPR/Cas systems to develop numerous visual biosensors,which not only significantly enhance signal output but also substantially reduce detection time and cost.This re-view focuses on five nanomaterials-graphene oxide(GO),gold nanoparticles(AuNPs),MoS2 nanosheets,metal-organic frameworks(MOFs),and quantum dots(QDs)—that have been exten-sively integrated with CRISPR/Cas systems in recent years.We systematically summarize their distinct physical characteristics and specific applications in CRISPR/Cas-based pathogen detection,followed by a concise comparison of the advantages and limitations of different methodologies.Fi-nally,we discuss the prospects for nanomaterials in CRISPR/Cas detection systems,aiming to pro-vide a valuable reference for advancing molecular diagnostics of pathogenic microorganisms.
