Adipose stem cells regulate the CHOP/Bcl-2 pathway to alleviate acute pancreatic injury in dogs
10.16303/j.cnki.1005-4545.2025.11.25
- VernacularTitle:脂肪干细胞调控CHOP/Bcl-2通路缓解犬急性胰腺损伤
- Author:
Mingzhen CHEN
1
;
Ruxin DING
1
;
Zhiying WAN
1
;
Yansong GE
1
;
Jiasan ZHENG
1
Author Information
1. 黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319
- Publication Type:Journal Article
- Keywords:
acute pancreatitis;
adipose derived stem cells;
endoplasmic reticulum stress;
CHOP;
ap-optosis;
dog
- From:
Chinese Journal of Veterinary Science
2025;45(11):2500-2506
- CountryChina
- Language:Chinese
-
Abstract:
Acute pancreatitis(AP)is a common and severe digestive disease in dogs,characterized by a high recurrence rate and complications that significantly impact canine health.This study in-vestigates the mechanism by which adipose-derived stem cells(ADSCs)regulate the CHOP/Bcl-2 pathway to mitigate acute pancreatic injury in dogs.Twenty Beagle dogs were randomly divided in-to four groups:a Sham group,an AP model group,an ADSCs treatment group,and a conditioned medium(CM)treatment group.The AP model was established by injecting a mixed solution of trypsin and sodium taurocholate into the AP model group.The ADSCs treatment group received intravenous injections of ADSCs immediately following surgery,while the CM treatment group re-ceived conditioned medium preparations.Pancreatic tissue was collected 24 hours post-surgery,and changes in the CHOP/Bcl-2 pathway were assessed using histopathology,transmission electron microscopy,western blotting,fluorescence quantification,immunofluorescence,and TUNEL stai-ning.The results indicated that AP induced significant interstitial edema,hemorrhage,inflammato-ry cell infiltration,chromatin contraction,and endoplasmic reticulum swelling,the expression lev-els of GRP78 and CHOP were found to be elevated,whereas the expression of Bcl-2 was downreg-ulated.Additionally,the expression levels of BAX,JNK,Caspase-3,and Caspase-12 increased,leading to an increased rate of cell apoptosis.Such changes result in an elevated apoptosis rate and a further decrease in Bcl-2 expression.Both ADSCs and CM therapy were found to alleviate the path-ological damage in pancreatic tissue,resulting in downregulation of CHOP and apoptosis-related markers,upregulation of Bcl-2,and a reduction in the apoptosis rate.The results of this study sug-gest that ADSCs may mitigate acute pancreatic injury induced by trypsin and sodium taurocholate in AP dogs by modulating the CHOP/Bcl-2 pathway.Targeted modulation of the transcriptional activity of CHOP may offer novel therapeutic approaches for AP.
