Effect of vitrification and repeated cryopreservation on epigenetics and developmental potential of early embryos
10.3760/cma.j.cn101441-20250326-00149
- VernacularTitle:玻璃化冷冻及冷冻次数对早期胚胎表观遗传及发育潜能的影响
- Author:
Linyan YANG
1
;
Zhiqiang WANG
;
Yali NI
Author Information
1. 甘肃中医药大学第一临床医学院，兰州 730000
- Publication Type:Journal Article
- Keywords:
Gene expression regulation;
Vitrification;
Epigenetic modifications;
Embryo developmental potential
- From:
Chinese Journal of Reproduction and Contraception
2025;45(9):948-953
- CountryChina
- Language:Chinese
-
Abstract:
Vitrification is a critical embryo cryopreservation technique extensively employed in assisted reproductive technology clinical practice. Recent evidence from animal models and clinical studies indicates that vitrification may compromise embryonic developmental competence by inducing epigenetic alterations, including dysregulated DNA methylation patterns, aberrant histone modifications, and subsequent disruptions in gene expression regulation. This review systematically summarizes recent advances from both animal and human studies, highlighting molecular mechanisms underlying vitrification-induced changes in embryonic developmental potential, with particular emphasis on epigenetic and developmental consequences of repeated vitrification. The aim of this review is to provide reproductive genetic insights and theoretical references to facilitate standardized clinical application of vitrification procedures and comprehensive assessment of embryo safety.
