Screening and identification of African swine fever virus M1249L interacting fac-tors based on yeast two-hybrid system
10.16303/j.cnki.1005-4545.2025.11.01
- VernacularTitle:基于酵母双杂交系统筛选与鉴定非洲猪瘟病毒M1249L互作因子
- Author:
Shuai CUI
1
;
Yang WANG
;
Shiyu CHEN
;
Yajun JIANG
;
Lichun FANG
;
Zhongbao PANG
;
Xiaoyu GUO
;
Hong JIA
;
Hongfei ZHU
Author Information
1. 中国农业科学院北京畜牧兽医研究所,北京 100193
- Publication Type:Journal Article
- Keywords:
African swine fever virus;
yeast two-hybrid;
capsid protein M1249L;
interacting protein;
protein analysis
- From:
Chinese Journal of Veterinary Science
2025;45(11):2301-2308
- CountryChina
- Language:Chinese
-
Abstract:
To explore the interaction between ASFV capsid protein M1249L and host from the host cellular perspective,M1249L was selected for constructing the bait plasmid(pGBKT7-M1249L)to screen the bone marrow-derived macrophages(BMDMs)cDNA library.After again co-transform and sequence alignment,20 candidate interacting host proteins were screened,such as IL-1β,CTSB and DNAJA3.And then,co-immunoprecipitation assay was performed to verify the interaction be-tween M1249L and host proteins.GO ontology(GO)and KEGG pathway enrichment analyses re-vealed that biological regulation,cellular communication and response to stimulus and others were enriched in biological processes.And these host proteins could share some pathways,including toll-like receptor signaling pathway and Nod-like receptor signaling pathway.Therefore,the results provides the theoretical basis for further research on the mechanism of ASFV M1249L in viral in-fection and immune regulation.
