Clinical value of serum tRNA-Gly-GCC-1-1 in the diagnosis and therapy of Sj?gren's syndrome
10.13602/j.cnki.jcls.2025.01.05
- VernacularTitle:血清tRNA-Gly-GCC-1-1在干燥综合征诊断中的临床价值
- Author:
Bo SHI
1
;
Fei CHEN
;
Wenjing LIU
;
Ping YANG
;
Zhiyang LI
Author Information
1. 南京医科大学鼓楼临床医学院 & 南京鼓楼医院检验科,南京 210011;南京市江宁中医院检验科,南京 211100
- Publication Type:Journal Article
- Keywords:
Sj?gren's syndrome;
RNA sequencing;
tsRNA;
biological marker
- From:
Chinese Journal of Clinical Laboratory Science
2025;43(1):25-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect and identify the differentially expressed tsRNA in the serum of patients with Sj?gren's syndrome(SS),and preliminarily explore their potential application value in the diagnosis of SS.Methods The serum samples from SS patients diagnosed at the Rheumatology Department of Nanjing Drum Tower Hospital and healthy controls(HCs)matched age and gender were collected,and the differentially expressed tsRNA were screened by small RNA sequencing.Fluorescence quantitative real-time PCR(qRT-PCR)was used to valid the sequence data.The receiver operating characteristic(ROC)curve was used to evaluate the diagnos-tic efficiency of tRNA-Gly-GCC-1-1.KEGG and GO analysis were used to predict the potential mechanism of tRNA-GLy-GCC-1-1 in the occurrence and development of SS.Results Compared with HCs(13.34[10.45,18.74]fmol/L),the levels of serum tRNA-Gly-GCC-1-1 in SS patients(8.82[7.26,12.20]fmol/L)were significantly reduced(P＜0.000 1),and its area under the ROC curve(AUCROC)in distinguishing SS patients from HCs was 0.766(95%CI:0.671-0.861).When the cut-off value was 9.97 fmol/L,its sensitivity and specificity were 83.33%and 64.58%,respectively.Bioinformatics analysis showed that serum tRNA-Gly-GCC-1-1 might be involved in the regulation of signaling pathways such as PI3K-Akt.Conclusion Serum tRNA-Gly-GCC-1-1 in SS patients is significantly down-regulated,which has potential clinical application value in assisting the diagnosis of SS.
