Intraperitoneal injection of hydrogen-rich saline promotes reverse cholesterol transport and decreases plasma lipid levels in golden hamster with hyperlipidemia
10.20039/j.cnki.1007-3949.2024.12.004
- VernacularTitle:腹腔注射富氢生理盐水促进高脂血症金黄地鼠胆固醇逆向转运和血脂水平降低
- Author:
Wei CHEN
1
;
Xincheng WANG
;
Yuan CHEN
;
Shucun QIN
Author Information
1. 山东第一医科大学第二附属医院;山东第一医科大学临床与基础医学院;山东第一医科大学泰山氢生物医学研究院,山东省泰安市 271000
- Publication Type:Journal Article
- Keywords:
hydrogen-rich saline;
gold hamster;
hyperlipidemia;
reverse cholesterol transport;
plasma lipids
- From:
Chinese Journal of Arteriosclerosis
2024;32(12):1041-1050
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of intraperitoneal injection of hydrogen-rich saline(HRS)on reverse cho-lesterol transport in golden hamsters with high fat diet.Methods The male golden hamsters were divided into three groups:chow diet group,high-fat diet group,and high-fat diet+HRS group.After 12 weeks of high-fat diet feeding and HRS administration,macrophages labeled with 3 H-cholesterol were injected into hypercholesterolemic golden hamsters,and then radioactivity in blood,liver,bile,and feces was measured.RT-qPCR and Western blot were used to assess the transcription and protein expression levels of cholesterol reverse transport-related genes in liver tissue.Results Long-term feeding with a high-fat diet induced significant hyperlipidemia and liver lipid accumulation in golden hamsters.Com-pared with the high-fat diet group,after HRS intervention,the body mass of golden hamsters decreased(P＜0.01),plasma TC and LDLC significantly decreased(P＜0.05),TG slightly decreased(P=0.11),HDLC significantly increased(P＜0.01),oxidative stress index MDA in plasma and liver significantly decreased(P＜0.05 or P＜0.01),antioxidant index glutathione(GSH)significantly increased(P＜0.01),liver weight/body weight ratio slightly decreased(P=0.05),TC and TG in liver decreased by 10.8％(P=0.05)and 20.1％(P＜0.01),respectively.Liver steatosis was significantly relieved,but there was no significant change in inflammatory factor levels.In isotopic tracing,high-fat diet fed golden hamsters treated with HRS showed decreased 3H radioactivity in plasma at 24 and 48 hours by 16.5％(P＜0.01)and 8.9％(P＜0.05)respectively,while increased 3H radioactivity was observed in liver,bile,and feces by 1.2-fold(P＜0.05),1.2-fold(P=0.08),and 1.1-fold(P=0.08)respectively,compared to those fed a high-fat diet alone.Fur-thermore,RT-qPCR and Western blot analyses of liver tissue demonstrated that HRS intervention resulted in a decrease of CD36,scavenger receptor-B1(SR-B1),and low density lipoprotein receptor(LDLR)protein levels by 39.5％(P＜0.05),40.5％(P＜0.01),and 28.0％(P＜0.01)respectively,an increase of ATP-binding cassette transporter G5(ABCG5)and sterol regulatory element-binding protein(SREBP2)protein levels by 1.5-fold(P＜0.05)and 1.3-fold(P＜0.01),and an increase of mRNA levels of ATP-binding cassette transporter A1 and G8 by 2.9-fold(P＜0.05)and 3.2-fold(P＜0.01)respectively in high-fat diet-fed hamsters.Conclusions Intraperitoneal injection of HRS promotes reverse cholesterol transport in high-fat diet-fed golden hamsters and exerts lipid-lowering effects.Additionally,intraperitoneal injection of HRS may alleviate hepatic lipid accumulation by inhibiting hepatic lipid uptake and promoting cholesterol excretion from liver.
