Effects of Qilou Pill-Containing Serum on P53 Signalling Pathway in Human Hepatocellular Carcinoma HepG2 Cells
- VernacularTitle:芪楼丸含药血清对人肝癌HepG2细胞P53信号通路的影响
- Author:
Yu LONG
1
;
Li WU
;
Xiaoping HUANG
;
Bo LI
;
Mei WEI
Author Information
- Publication Type:Journal Article
- Keywords: Qilou pill; P53 pathway; HepG2; Proliferation; Apoptosis
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(12):3153-3159
- CountryChina
- Language:Chinese
- Abstract: Objective To study the anticancer effects of Qilou Pill-containing serum on human hepatocellular carcinoma HepG2 cells in vitro and its mechanism.Methods Human hepatoma HepG2 cells were cultured in vitro and divided into the control group and the low,medium and high dose groups of Qilou Pills,and each group was treated with corresponding serum containing drugs.The proliferation and apoptosis of HepG2 cells were detected by CCK-8 method and Annexin V-FITC/PI double staining.The mRNA and protein expression levels of P53,P21,Caspase-3,Bax and Bcl-2 were detected by real-time fluorescence quantitative PCR and Western blot,respectively.Results ① Qilou Pill-containing serum inhibited the proliferation of human hepatoma HepG2 cells in a concentration and time dependent manner,with statistical significance compared with the control group(P<0.01).②Qilou Pill drug-containing serum could promote the apoptosis of human hepatoma HepG2 cells,and the promoting effect was more significant with the increase of drug concentration.The difference between medium and high dose groups and control group was statistically significant(P<0.01),while the difference between low dose group was not significant(P>0.05).③Compared to the control group,the mRNA expressions of P53,P21,Caspase-3 and Bax in Qilou Pill serum increased with the increase of drug dose,while the Bcl-2 mRNA showed the opposite trend,with statistical significance(P<0.05 or P<0.01).④Compared with the control group,the protein expression levels of P53,P21,Caspase-3 and Bax in Qilou Pill-containing serum dose groups were increased,and showed an increasing trend with the increase of drug dose(P<0.05 or P<0.01).On the contrary,the expression level of Bcl-2 protein decreased gradually with the increase of drug dose,and the difference was statistically significant(P<0.01).Conclusion Qilou Pills can inhibit the proliferation of human hepatoma HepG2 cells and promote their apoptosis by participating in the transmission of P53 signalling pathway,so as to play a role in the treatment of hepatoma.
