Effect and Mechanism of Lobetyolin on Cholesterol Metabolism in HepG2 Cells
- VernacularTitle:党参炔苷对HepG2细胞胆固醇代谢的影响及机制研究
- Author:
Ruiling YANG
1
;
Qiang CHEN
;
Guibin XIONG
;
Xiaoming ZHANG
;
Jie LI
;
Faming WU
;
Chengxin SUN
Author Information
- Publication Type:Journal Article
- Keywords: Lobetyolin; HepG2 cells; Metabolism; Cholesterol; PPARγ/CYP7A1
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(1):154-160
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the mechanism of lobetyolin's intervention in HepG2 cells abnormal cholesterol metabolism.Methods This study used oleic acid(OA)stimulation of HepG2 cells as a model.MTT assay,oil red O staining,biochemical kit assay,qRT-PCR assay,Western blot assay and NBD labeled cholesterol effection assay were used to study the effect of lobetyolin on cholesterol metabolism in HepG2 cells.Results The results showed that lobetyolin could reduce the content of lipid drops,the levels of triglyceride(TG)and total cholesterol(TC)in HepG2 cells stimulated by OA,increase cholesterol effection rate,and up-regulate cytochrome 7A1(CYP7A1),liver x receptor α(LXRα),ATP-binding cassette transporter A1(ABCA1),peroxisome proliferator-activated receptor(PPARγ)and other mRNA or protein expression levels.However,combined intervention with PPARγ antagonist Mifobate(SR-202)can significantly inhibit the promoting effect of lobetyolin on cholesterol metabolism in HepG2 cells.Conclusion This study revealed that lobetyolin can improve the cholesterol effection rate of HepG2 cells stimulated by OA and promote cholesterol catabolism,and the mechanism of action may be related to the regulation of PPARγ/CYP7A1 pathway.
