Cloning and Prokaryotic Expression of Three Flavonoid Glycoside Synthesizing UGT Genes of Chrysanthemum indicum.L
10.11842/wst.20240601003
- VernacularTitle:野菊中三个黄酮苷合成UGT基因克隆及原核表达
- Author:
Zhiwei QIN
1
;
Jiahao LIAO
1
;
Jingjing ZHANG
1
;
Yifei LIU
1
;
Zhigang HU
1
;
Jingjing LIU
1
Author Information
1. 湖北中医药大学药学院 武汉 430065
- Publication Type:Journal Article
- Keywords:
Chrysanthemum indicum.L;
UGT;
Prokaryotic expression;
Flavonoid
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(1):56-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective The three UGT genes involved in the synthesis of flavonoid glycosides in the wild chrysanthemum were cloned,fused and expressed in prokaryotic system,which provided a basis for further study of the functions of these genes in the synthesis of flavonoid compounds.Methods three candidate UDP-glycosyltransferase(UGT)family genes were successfully cloned from flower tissue cDNA of diploid Chrysanthemum indicum.L in Hubei,namely:CiUGT1(1425 bp),CiUGT2(1311 bp)and CiUGT3(1314 bp)encoded opal fraction of 51.72,48.08 and 48.28 kDa,respectively.After the physical and chemical properties of the protein were analyzed,the recombinant plasmid was constructed by seamless Klon technology,and the recombinant proteins of CiUGT2 and CiUGT3 were successfully expressed in Escherichia coli.Results SDS-PAGE analysis showed that the purified CiUGT2 and CiUGT3 protein samples were about 0.2 mg/mL and 1.4 mg/mL,respectively.Conclusion Two candidate UGTs were obtained to catalyze the synthesis of flavonoid 7-O-glucosides in Chrysanthemum indicum.L,which provide the theoretical basis for the molecular mechanism of flavonoid glycosides biosynthesis in the capitulum of Chrysanthemum indicum.L.
