Effects of Exogenous Hormones Combined with Thermotropic Stratification on Post-Ripening and Germination of Coptis chinensis Seeds
10.11842/wst.20240725004
- VernacularTitle:外源激素结合变温层积对黄连种子后熟与萌发的影响
- Author:
Siyu WANG
1
;
Guofeng LI
;
Siyu YANG
;
Shilin CHEN
;
Di LIU
;
Yifei LIU
Author Information
1. 湖北中医药大学药学院 武汉 430065
- Publication Type:Journal Article
- Keywords:
Coptis chinensis seeds;
Gibberellin;
Variable temperature stratification;
Afterripening;
Germinate
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(1):14-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this study,exogenous hormones combined with variable temperature stratification were used to relieve seed dormannia of Coptis chinensis and promote seed germination,and the effects of gibberellin on the physiological,biochemical and embryonic morphological changes of C.chinensis seeds during post-mature germination were further evaluated.Methods Using orthogonal test,different exogenous hormones(GA3,6-BA,NAA,ABA)and different temperature changing stratification(20℃/4℃)were set up,and the germination index(germination rate and split rate)of C.chinensis seeds were statistically compared.The activity of antioxidant enzymes(peroxidase POD,catalase CAT),the content of malondialdehyde(MDA)and endogenous hormones(GA,IAA,ABA)during the germination of C.chinensis seeds under optimal treatment were measured.The microscopic structure of the seeds was observed by paraffin embedding section.Results The results of range analysis showed that the best budding method was:100 mg·L-1 of gibberellin for 12 h,20℃for 60 day,4℃for 10 day.Physiological and biochemical index analysis showed that 100 mg·L-1 exogenous gibberellin treatment promoted the endogenous GA content to reach the peak in advance,maintained the POD and CAT contents at a higher level,and maintained the malondialdehyde(MDA)content at a lower level.The transformation from torpedo embryo to cotyledonous embryo was observed by electron microscope during postmature germination.Conclusion Exogenous gibberellin combined with variable temperature stratification treatment could significantly accelerate seed germination of C.chinensis,mainly by promoting seed embryo morphological development,affecting the contents of GA,POD and CAT,and rapidly relieving seed dormancy to promote seed germination.Compared with natural conditions,the germination period could be shortened by about six months.
