Ganoderma Lucidum Extract Nano-emulsion:Preparation,Characterization and Pharmacodynamics Study in Anti-skin Aging
10.3870/j.issn.1004-0781.2025.12.022
- VernacularTitle:灵芝糖萜纳米乳的制备与表征及抗皮肤衰老药效学研究
- Author:
Jia LU
1
;
Hailun HAN
;
Mengfei WU
;
Donghua YU
;
Zihua XUAN
;
Jing ZHANG
;
Can PENG
Author Information
1. 安徽中医药大学,新安医学与中医药现代化研究所,合肥 230012;安徽中医药大学药学院,合肥 230
- Publication Type:Journal Article
- Keywords:
Ganoderma lucidum extract;
Nano-emulsion;
Preparation technology;
Quality evaluation;
Anti-skin aging;
Pharmacodynamic evaluation
- From:
Herald of Medicine
2025;44(12):2030-2038
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the prescription,preparation,quality evaluation of Ganoderma lucidum extract nano-emulsion(GLEN)and study its anti-skin aging effect.Methods The emulsifier and oil phase of the nano-emulsion were preliminarily screened by solubility method.The pseudo-ternary phase diagram method was used to screen the nano-emulsions,and the prepared GLEN were evaluated for quality in terms of the appearance,particle size,structure,drug loading,stability and skin safety.A mouse skin aging model was constructed by injecting D-galactose combined with ultraviolet radiation.GLEN was applied to the skin at the modeling site and investigated for their anti-skin aging efficacy.Results The optimal GLEN prescription was 30%polyoxymethylene castor oil,14.4%glycerol,11.1%medium chain triglycerides,and 44.5%water.GLEN was a dark brown and transparent liquid at room temperature,O/W type nano-emulsion with an average particle size(32.20±2.89)nm,polydispersity coefficient 0.38±0.03,zeta potential value(-4.05±0.27)mV.The content of Ganoderma lucidum polysaccharides was(36.35±0.55)mg·mL-1,and the content of Ganoderma lucidum triterpenes was(5.99±0.46)mg·mL-1,which possessed good stability.Pharmacodynamic experiments showed compared with the model group,the skin scores of mice in the GLEN group significantly improved(P﹤0.05),the water content increased(P﹤0.01),and the contents of SOD,GSH-PX and Hyp increased(P﹤0.05),while the content of MDA was decreased significantly(P﹤0.01).HE,Masson and Sirius crimson staining further indicated that the pathological changes in mice were alleviated to varying degrees in GLEN group.And GLEN had high safety for skin,could be applied to human body.Conclusion In this study,the prepared GLENs were homogeneous in appearance,stable in nature,and exhibited significant anti-aging effects on mouse skin.
