Preliminary study on predicting early embryonic developmental potential by metabolomics analysis of day 3 embryo culture medium
10.3760/cma.j.cn101441-20240914-00342
- VernacularTitle:第3天胚胎培养液代谢组学分析预测早期胚胎发育潜能的初步研究
- Author:
Xuehua CHEN
1
;
Jinhui SHU
;
Kejian SUN
;
Hong ZHOU
;
Xiaojing FAN
;
Caizhu WANG
Author Information
1. 广西中医药大学附属瑞康医院妇产科，南宁 530011
- Publication Type:Journal Article
- Keywords:
Metabolomics;
Reproductive technology, assisted;
Embryo development potential;
Embryo culture medium;
Liquid chromatograph-mass spectrometer
- From:
Chinese Journal of Reproduction and Contraception
2025;45(4):380-389
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the differences of metabolomic profiles in day 3 (D3) culture medium of embryos with varying developmental potentials, in order to provide a theoretical foundation for the establishment of embryo selection technology platform using metabolomics.Methods:Eight patients who received in vitro fertilization and embryo transfer (IVF-ET) treatment at Reproductive Medicine Center of Guangxi Zhuang Autonomous Region Maternal and Child Health Hospital between November 13 and December 5, 2023 were selected as the study subjects. The D3 culture medium from patient embryos was collected and divided into high-quality blastocysts ( n=42), non-high-quality blastocysts ( n=33), and embryos that failed to form blastocysts (non-formation group, n=43) according to the formation of day 5 blastocysts. High-performance liquid chromatography-mass spectrometry was employed to perform non-targeted metabolomic analysis in the D3 culture medium from three distinct groups. Results:1) The metabolites in D3 culture medium of embryos with varying developmental potentials exhibit significant differences. Specifically, 79 differential metabolites were identified between the blastocyst formation group and the non-blastocyst formation group (all P<0.05); additionally, 73 differential metabolites were found between the high-quality blastocyst group and the non-high-quality blastocyst group (all P<0.05). 2) The area under the receiver operating characteristic curve of significantly differential metabolites for predicting potential of D3 embryo blastocyst formation and high-quality blastocyst formation were both greater than 0.9, demonstrating excellent predictive performance. 3) KEGG pathway enrichment analysis revealed that differential metabolites associated with blastocyst formation potential were primarily enriched in pathways including D-amino acid metabolism, glycine-serine-threonine metabolism, arginine biosynthesis, and histidine metabolism ( P<0.05). For high-quality blastocyst formation, the differential metabolites were predominantly enriched in pathways related to tryptophan metabolism, D-amino acid metabolism, serotonergic synapses, and protein digestion and absorption ( P<0.05). Conclusion:Embryos with different developmental potentials have significantly different metabolic profiles, and it is feasible to predict the developmental potential of D3 embryos by metabolomics analysis.
