Loureirin A Inhibits Neutrophil Infiltration and Improves Cerebral Vascular Damage in Photothrombotic Stroke Mice
- VernacularTitle:龙血素A抑制中性粒细胞浸润改善光栓塞脑卒中模型小鼠脑血管损伤
- Author:
Liqing LI
1
;
Zhao ZHANG
;
Naihong CHEN
Author Information
- Publication Type:Journal Article
- Keywords: Ischemic stroke; Loureirin A; Neutrophil; ROS; Vascular injury
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(12):3246-3255
- CountryChina
- Language:Chinese
- Abstract: Objective This study aims to explore whether Loureirin A can mitigate neutrophil-related pathology and vascular damage in a photothrombotic stroke model.Methods C57BL/6J mice were divided into three groups:sham-operated,model,and Loureirin A administered(Model+Loureirin A,10 mg·kg-1).A mouse model of focal cerebral ischemia was established using photothrombosis(PT),and the administration of Loureirin A commenced one day post-surgery via intraperitoneal injection for three consecutive days.Neurological function was assessed in the mice 24 h post-PT modeling,while the infarction rate was evaluated through TTC staining three days post-PT modeling.MPO-positive cell infiltration in the peri-infarct cortex was detected by immunohistochemistry.The reactive oxygen species(ROS)content was observed via immunofluorescence staining.Cerebral blood flow in mice was measured by laser speckle imaging.The expression of tight junction proteins ZO-1(Zonula occludens-1),Occludin,and matrix metalloproteinase-9(MMP-9)was detected by immunoblotting.Vascular permeability was assessed by 40 kDa FITC-Dextran,and peri-infarct vascular density was detected by 2000 kDa FITC-Dextran.Results Loureirin A markedly decreased the cerebral infarction rate and the extent of neurological functional impairment,and enhancing blood perfusion.Pathological examinations revealed that Loureirin A lessened neutrophil infiltration,reduced intracellular ROS,and increased the protein expression of ZO-1 and Occludin,which are critical components of the blood-brain barrier.Concurrently,it downregulated Matrix Metalloproteinase-9(MMP-9).Furthermore,Loureirin A diminished the leakage of extracellular 40 kDa dextran,bolstered peri-infarct cerebral vascular perfusion,and mitigated vascular injury.Conclusion Loureirin A can inhibit neutrophil infiltration and vascular injury after cerebral ischemia.
