Exploring the Mechanism of Yiwei Decoction on Hypothalamus of Premature Ovarian Insufficiency Rats Based on Transcriptomics
- VernacularTitle:基于转录组学探究益胃汤干预POI大鼠下丘脑的作用机制
- Author:
Hong LEI
1
;
Weisen FAN
;
Xuan LI
;
Pu CHEN
;
Yingjie ZHANG
Author Information
- Publication Type:Journal Article
- Keywords: Yiwei decoction; Premature ovarian insufficiency; Transcriptome sequencing; Adaptive immune response; Differentially expressed genes
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(12):3016-3024
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the mechanism of Yiwei Decoction on hypothalamus of rats with premature ovarian insufficiency(POI)by transcriptome sequencing(RNA-seq)technology.Methods The rat model of POI was established by intraperitoneal injection of CTX.The 30 rats were randomly divided into Yiwei Decoction group(group A),model group(group B)and control group(group C).The rats in the group B and group C were intragastrically administered with normal saline,while those in the group A were intragastrically administered with 9 mL·kg-1·d-1 Yiwei Decoction for four consecutive weeks.The gene expression profiles in the hypothalamus of rats in each group were detected,and the target differentially expressed genes(DEGs)were screened.GO,KEGG,GSEA and PPI analyses of DEGs were performed.Western blot was used to detect the protein expression levels of CXCR1,IL16 and IL12rb2 in rat hypothalamus.Results There were 142 DEGs in group A and B,of which 98 genes were up-regulated and 44 genes were down-regulated.GO analysis results showed that DEGs was mainly concentrated in T-helper cell differentiation regulation and cytokine-mediated signaling pathways.KEGG analysis showed that DEGs in group A and B were mainly enriched in cytokine-cytokine receptor interactions,Th1 and Th2 cell differentiation,B cell receptor signaling pathway.The DEGs in group B and C were mainly rich in PI3K-Akt signaling pathway and Th17 cell differentiation.GSEA analysis showed that Yiwei Decoction may play a role by downregulating the ABC transporter pathway.PPI analysis showed that the core genes were Gata3,Cd22,and Cd2.The results of Western blot showed that compared with group C,the expression levels of CXCR1 and IL16 proteins in the hypothalamus of group B were high,the expression of IL12rb2 protein in the hypothalamus of group B was low.Compared with group B,the expression levels of CXCR1 and IL16 proteins in the hypothalamus of group A were low,the expression of IL12rb2 protein in the hypothalamus of group A was high.Conclusion This study revealed the mechanism of Yiwei Decoction's intervention in the hypothalamus of POI rats,and further experiments are needed to verify the specific roles of these key genes and pathways.
