NLRP3-COX-2 signaling pathway activating microglia mediates breast cancer-related depression
- VernacularTitle:NLRP3-COX-2信号通路激活小胶质细胞介导乳腺癌并发抑郁症
- Author:
Song YANG
1
;
Ying HE
;
Yuan-shan HAN
;
Man-shu ZOU
;
Hai-xia HE
;
Yu-HONG WANG
Author Information
- Publication Type:Journal Article
- Keywords: breast cancer-related depression; NL-RP3; COX-2; microglia; neuron; neuroinflammation
- From: Chinese Pharmacological Bulletin 2025;41(9):1754-1761
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effects of breast cancer-related depression model on microglial activa-tion and neuronal function via the NLRP3-COX-2 sig-naling pathway.Methods In the cell experiment,BV2 and HT22 cells were co-cultured to observe the polarization of BV2 cells and the injury of HT22 cells.In the animal experiment,behavioral experiments were used to evaluate the depressive behaviors of mice.HE staining and tumor weight were used to evaluate the changes of axillary tumors.ELISA was used to detect the contents of CA153,CA125,CEA,as well as the changes in the contents of 5-HT,DA and others in the serum and hippocampus.RT-qPCR and immunohisto-chemistry were used to detect the mRNA and protein expression of IL-1 β,IL-18 and other indicators,re-spectively.Immunofluorescence was used to detect CD1 1b and Iba-1.Nissl staining and transmission elec-tron microscopy were used to observe the hippocampal neurons.Results The results of cell experiments showed that compared with the model group,the pro-tein contents and mRNA expression levels of NLRP3,COX-2,and IL-1 β in the NLRP3 knockdown group were all decreased.The results of animal experiments showed that compared with the model group,the be-havioral performance of the experimental group was im-proved,and the mRNA and protein expression levels of IL-1 β,IL-18,and NLRP3 were decreased.Conclu-sion BCRD may mediate the polarization of microglia and the injury of hippocampal neurons through the NL-RP3-COX-2 signaling pathway.
