Study on effects of icariin on chronic ulcerative colitis in mice
- VernacularTitle:淫羊藿苷对慢性溃疡性结肠炎的改善作用
- Author:
Cai-xia XUE
1
;
Hong-yue LI
;
Jin LI
;
Shi-yu ZHAO
;
Jian-lin YANG
;
Yao-yan DUN
;
Zheng-guo XIONG
Author Information
- Publication Type:Journal Article
- Keywords: chronic ulcerative colitis; icariin; M1 type macrophages; MAPK signaling pathway; M2 macro-phages; fibrosis
- From: Chinese Pharmacological Bulletin 2025;41(9):1743-1753
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of icariin(ICA)on chronic ulcerative colitis(UC)in mice.Methods Male SPF-grade C57BL/6J mice were ran-domly divided into the control group,model group,low-dose ICA group,and high-dose ICA group.Except for the control group,the rest of the mice were established as chronic UC models.Expressions of tight junction proteins,inflammatory factors,fibrosis markers,macro-phage markers,and MAPK signaling pathways in the colonic epithelium of each group were determined by Western blot,qPCR,immunohistochemistry;the degree of intestinal damage and fibrosis was detected by HE and Masson staining;the co-localization of different macrophage markers with related factors was examined by immunofluorescence.Results Treatment of ICA significantly improved the general condition of chronic UC mice,decreased the infiltration of M1-type macro-phages and the secretion of pro-inflammatory factors,inhibited the MAPK signaling pathway,reduced the co-localization expression of M1-type macrophages with the MAPK signaling pathway,and downregulated the infiltration of M2-type macrophages and the secretion of pro-fibrotic factors in the colon.Conclusion ICA mitigates the inflammatory damage and fibrosis of colon in chronic UC mice.
