Study on intestinal protection and mechanism of magnolol in neonatal rats with necrotizing enterocolitis
10.12360/CPB202412139
- VernacularTitle:厚朴酚对坏死性小肠结肠炎新生大鼠的肠道保护作用及机制
- Author:
Hai-yan FENG
1
;
Yue ZHANG
;
Mao XU
;
Kai-qi TAN
;
Yi WANG
;
Zhuo-lin CHEN
;
Yu-fei CHEN
;
Shao-xuan CHEN
;
Yang ZHAO
;
Cui LIU
Author Information
1. 湖北医药学院临床技能教学培训中心
- Publication Type:Journal Article
- Keywords:
necrotizing enterocolitis;
magnolol;
intes-tine;
microbiota;
16S rDNA;
NLRP3 inflammasome
- From:
Chinese Pharmacological Bulletin
2025;41(9):1728-1735
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the intestinal protection and possible mechanism of magnolol(MG)in newborn rats with necrotizing enterocolitis(NEC).Methods The rats were randomly divided into control group(Ctrl group),model group(NEC group)and treatment group(MG group).The NEC model was induced by hypoxia,cold stimulation,deep formula milk and LPS intragastric administration in 7-day-old rats for four days.They were killed after five days of treatment with MG(20 mg·kg-1).HE staining was used to observe the intestinal pathological injury.Western blot was used to detect the expressions of IL-1 β,TNF-α,NL-RP3,ASC,caspase-1 and tight junction protein in the distal ileum of rats.Colon contents were collected for 16S rDNA sequencing to understand the gut microbio-ta.Results MG improved the body mass and intesti-nal injury of NEC neonatal rats.The expressions of in-testinal IL-1β,TNF-α,NLRP3,ASC and caspase-1 proteins were down-regulated,and the expressions of Claudin,Occludin and ZO-1 proteins were up-regula-ted.16S rDNA showed that MG increased the diversity of intestinal flora,and at the phylum level,MG in-creased the abundance of firmicutes and bacteroides in NEC model,and decreased the abundance of pro-teobacteria.At the genus level,MG treatment in-creased the abundance of Lactobacillus,unclassified_Muribaculaceae,Racteroides,but decreased the abun-dance of Escherichia_Shigella,Rodentibacter and Fuso-bacterium.Conclusion MG intervention can protect the intestinal tract of NEC rats by potentially improving barrier function,and regulating the intestinal microbiota through the NLRP3/ASC/caspase-1 signaling pathway.
