Analysis of factors related to chromosomal abnormalities in abortion tissue of patients after IVF/ICSI assisted conception
10.3760/cma.j.cn101441-20240731-00276
- VernacularTitle:IVF/ICSI助孕后患者流产组织染色体异常相关因素分析
- Author:
Ming SHI
1
;
Chen ZHANG
;
Xin KANG
;
Huiyun YANG
;
Yang SHI
;
Xiaoxue LIU
;
Jing ZHANG
Author Information
1. 大连市妇女儿童医疗中心（集团）体育新城院区检验科，大连 116037
- Publication Type:Journal Article
- Keywords:
Chromosomal abnormalities;
Spontaneous abortion;
Reproductive technology, assisted
- From:
Chinese Journal of Reproduction and Contraception
2024;44(11):1170-1174
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the related factors of chromosome abnormality in abortion tissue of patients after in vitro fertilization/intracytoplasmic sperm injection and embryo transfer (IVF/ICSI-ET) and to establish a predictive model of chromosome abnormality, so as to provide more clinical treatment basis. Methods:This was a retrospective case-control study. The abortion tissues of 113 cases of IVF/ICSI assisted pregnancy (106 cases of IVF and 7 cases of ICSI) were collected from the Reproduction and Genetics Laboratory of Dalian Women and Children Medical Center (Group) from June 2022 to August 2023. The samples were detected by copy number variation sequencing (CNV-seq). According to the maternal age, the samples were divided into young pregnant women (<35 years old, n=45) group and elderly pregnant women (≥35 years old, n=68) group. The correlations between different maternal age, male age, duration of infertility, times of previous abortion, number of embryos transferred, gestational age and chromosome abnormalities were analyzed. Results:1) A total of 72 cases [63.72% (72/113)] of abnormal chromosomes were detected in the abortion tissues of patients with IVF/ICSI-assisted pregnancy, of which 61 cases [84.72% (61/72)] were numerically abnormal, most of them were trisomy (53 cases), structural abnormalities were found in 8 cases [11.11% (8/72)], and other complex abnormalities were found in 3 cases [4.17% (3/72)]. 2) The chromosome abnormality rate of abortion tissue in the young pregnant women [51.11% (23/45)] was lower than that in the elderly pregnant women [72.06% (49/68)], and the difference was statistically significant ( P=0.023). 3) In the abortion tissue of elderly pregnant women, univariate analysis showed that the age of the man and times of previous abortion were related to the occurrence of chromosome abnormalities ( P=0.004, P=0.008). 4) Multivariate logistic regression analysis showed that the age of the man and times of previous abortion were the independent risk factors for chromosomal abnormalities in elderly pregnant women ( OR=1.248, 95% CI: 1.064-1.464, P=0.006; OR=0.493, 95% CI: 0.287-0.848, P=0.011), the regression equation was logit (P)=0.222×male age-0.707×times of previous abortion-6.042, area under curve was 0.789, 95% CI: 0.673-0.905, and the maximum value of Jordan index was 0.489. Conclusion:The age of pregnant women was correlated with the occurrence of chromosomal abnormalities in abortion tissues. At the same time, in pregnant women aged 35 years or above, under the condition of fixed abortion rates caused by female factors, an increase in male age and a decrease in previous abortions will lead to an increase in the incidence of chromosomal abnormalities. The model is of good value in predicting chromosome abnormalities.
