Changes of the Gadolinium Deposition in Abdominal Organs After Administration of Different Types of Gadolinium Agents in Linear Gadolinium Deposition Model in Rats
10.3969/j.issn.1005-5185.2024.12.002
- VernacularTitle:线性钆沉积大鼠模型使用不同类型钆剂后腹部器官钆沉积的变化
- Author:
Xucong WANG
1
;
Jian LI
1
;
Bing CHEN
1
Author Information
1. 宁夏医科大学总医院放射科,宁夏 银川 750004
- Publication Type:Journal Article
- Keywords:
Gadolinium;
Contrast media;
Abdomen;
Mass spectrometry;
Rat;
Pathology
- From:
Chinese Journal of Medical Imaging
2024;32(12):1203-1208
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the changes of gadolinium deposition in abdominal organs after continuous injection of different types of gadolinium agents of a linear gadolinium deposition model in rats. Materials and Methods A preliminary model of linear gadolinium deposition was established in 28 male SD rats. Firstly,they were first injected with gadodiamide (linear gadolinium) 20 times (A single dose of 0.06 mmol/kg was given;4 times per week for 5 weeks). Randomly,they were divided into four groups:Omniscan (linear),gadolinium DOTA meglumine (macrocyclic),gadobutrol (macrocyclic) and hypertonic saline. The number and dose of injections were the same as before. At the 5th and 10th week after euthanasia,the kidney,liver and spleen tissues were extracted for inductively coupled plasma mass spectrometry to determine the concentration of gadolinium in the tissues,and the distribution of gadolinium particles in the tissues was observed and analyzed by transmission electron microscopy and X-ray energy spectrometer,and HE staining was performed for pathological observation. Results The gadolinium concentration in liver and spleen and kidney of all groups and the gadolinium concentration among all groups were statistically significant (H=12.82-21.10,P<0.01). The gadolinium deposition concentration in gadolinium diamine group was significantly higher than that in other groups (P<0.01),followed by macrocyclic group,and the lowest in hypertonic saline group. The concentration of gadolinium in kidney was significantly higher than that in liver and spleen (P<0.01). Transmission electron microscopy and X-ray energy spectrometer confirmed the deposition of gadolinium particles in tissues,and which were the most significant in gadodiamide group. HE staining showed that there were glomerular sclerosis and diffuse dilatation of renal tubules in all groups,which were significant in gadodiamide group. There was no significant histological injury or difference in liver and spleen among all groups. Conclusion In rats with linear gadolinium deposition,macrocyclic gadolinium-based contrast agent with gadolinium DOTA meglumine and gadobutrol have less gadolinium deposition in abdominal organs,it has little effect on kidney tissue damage and relatively safety. It provides an important reference for people who have used linear gadolinium agents in clinical practice to re-select gadolinium agents.
