Molecular detection of five intestinal protozoans in Chinese Milu deer populations in various regions of China
10.3969/j.issn.1002-2694.2024.00.180
- VernacularTitle:我国不同麋鹿种群肠道原虫的分子检测与鉴定
- Author:
Xing-long SONG
1
;
Chen-rong WANG
;
Hai-bo MA
;
Zhen-yu ZHONG
;
Jia-de BAI
;
Zhi-bin CHENG
;
Qing-yun GUO
;
Yun-fang SHAN
;
Hua JU
;
Cong-shan YANG
;
Qing-xun ZHANG
Author Information
1. 北京麋鹿生态实验中心,北京 100076;安徽农业大学动物科技学院,合肥 230036
- Publication Type:Journal Article
- Keywords:
Chinese Milu deer;
intestinal parasites;
protozoans;
molecular detection
- From:
Chinese Journal of Zoonoses
2024;40(12):1140-1145
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at understanding the Blastocystis,Enteroc ytozoon bieneusi,Cryptosporidium spp.,Gi-ardia duodenalis,and Pentatrichomonas hominis infection status in Chinese Milu deer(Elaphurus davidianus)in various prov-inces of China.A total of 81 fecal samples were collected from Beijing,Inner Mongolia,Hebei,and Hubei.PCR was used to detect the protozoans,and their subtypes and zoonoticity were determined through sequence and phylogenetic analyses.PCR re-sults indicated an infection prevalence of 40.74％,19.75％,and 8.64％for Blastocystis,E.bieneusi,and Cryptosporidium spp.,respectively,whereas G.duodenalis and P.hominis was not detected.Only one subtype of Cryptosporidium spp.(Cryptosporidium deer genotype)was detected.Four E.biene-usi genotypes were detected:HLJD-V,MWC-d1,BEB6,and CGC2.Five Blastocystis ST types were found:ST10,ST14,ST21,ST23,and ST25.Cryptosporidium spp.,E.bieneusi,and Blastocystis infections were prevalent,and zoonotic subtypes or genotypes of E.bieneusi and Blastocystis were i-dentified.The prevention and control of intestinal protozoa in Chinese Milu deer would support population health and is im-portant for public health.
