Whole genome sequencing and antimicrobial resistance of Listeria monocytogenes in retail fresh edible mushrooms in Fujian Province
10.3969/j.issn.1002-2694.2024.00.176
- VernacularTitle:福建省零售新鲜食用菌分离单核细胞增生李斯特菌全基因组测序及耐药分析
- Author:
Xue-jie LIU
1
;
Wei-wei CHEN
1
;
Gong-de LIN
1
;
Zhi-fang ZHANG
1
;
Tian-you ZHANG
1
Author Information
1. 福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福州 350012
- Publication Type:Journal Article
- Keywords:
fresh edible mushrooms;
Listeria monocytogenes;
whole genome sequencing
- From:
Chinese Journal of Zoonoses
2024;40(12):1152-1158
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the serogroups,ST types,virulence genes,and resistance characteris-tics of Listeria monocytogenes(Lm)isolates from fresh edible mushrooms in Fujian Province.The whole genomes of Lm isola-ted from fresh edible mushrooms in Fujian province were sequenced.BioNumeric7.6 software was used to assemble the sequen-cing data,and the serogroups,ST types,virulence genes,and drug resistance characteristics were further analyzed.Ten Lm strains were classified into three serogroups(six Ⅱb strains,three Ⅱa strains,and one Ⅱc strain),five ST types(four ST5 strains;three ST8 strains;and one strain each for ST87,ST9,and ST429).CC5 and CC8 were the dominant clone groups.All Lm carried six virulence genes(actA,hly,mpl,plcA,plcB,and prfA)on LIPI-1,as well as internal genes(inlA,inlB,inlC,inlE,inlF,inlH,inlJ,and inlK).Some strains carried inlD,inlG,and inlL.Neither strain carried the gene on LI-PI-3,and two strains carried some genes on LIPI-4.The resistance rate of Lm to cefoxitin was 100％,and the resistance rates to oxacillin and clindamycin were both 30％.Five strains were resistant to two or more antibiotics,one ST87 strain had a resistance spectrum of OXA-CLI-CIP-FOX,two ST8 strains had a resistance spectrum of CLI-FOX,and two ST5 strains had a re-sistance spectrum of OXA-FOX.Prediction of antibiotic resistance genes revealed that all ten Lm carried fosX,mprF,norB,sul,and Lmo0919 antibiotic resistance genes.According to cgMLST a-nalysis,strains of Lm clustered on five separate evolutionary branches,each containing one ST type.No allelic differences were identified among the three ST8 strains and the four ST5 strains of Lm,thus indicating that they belonged to the same clone and could sustainably exist in fresh edible mushrooms.Lm strains isolated from retail fresh edible mushrooms in Fujian Province pose a potential risk human infection,and the drug re-sistance status is concerning.In particular,a highly virulent multi-drug resistant strain found in flammulina velutipes may pose a potential threat to public health and should be alerted.
