Relationship between angiotensin Ⅱ type 1 receptor autoantibody levels in follicular fluid and follicular development in patients with PCOS
10.3760/cma.j.cn101441-20240321-00100
- VernacularTitle:PCOS患者卵泡液中血管紧张素Ⅱ-1型受体自身抗体与卵泡发育的关系研究
- Author:
Yana YU
1
;
De SONG
;
Guifang YANG
;
Yao CHEN
;
Yinan ZHANG
;
Xuemin LI
;
Jiahui ZHOU
;
Yuhui SHI
;
Xiaoli YANG
Author Information
1. 太原市中心医院辅助生殖中心，太原 030000
- Publication Type:Journal Article
- Keywords:
Polycystic ovary syndrome;
Follicular fluid;
Angiotensin Ⅱ type 1 receptor autoantibody;
Follicular development
- From:
Chinese Journal of Reproduction and Contraception
2024;44(9):922-929
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the presence of angiotensin Ⅱ type 1 receptor autoantibodies (AT1-AA) in follicular fluid, and to investigate the correlation between AT1-AA levels and follicular development in follicular fluid of infertile patients with polycystic ovary syndrome (PCOS).Methods:A case-control study was conducted on 182 infertile patients who underwent in vitro fertilization/intracytoplasmic sperm injection and embryo transfer (IVF/ICSI-ET) at the Center for Reproductive Medicine of Taiyuan Central Hospital from June 2021 to October 2022. There were 80 cases in the fallopian tube factor group (tubal factor group) and 102 cases in PCOS infertility group (PCOS group). Univariate analysis was performed for the general data, ovulation induction indexes and embryonic laboratory indexes of the two groups. Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of AT1-AA in follicular fluid on the day of oocyte retrieval. Pearson or Spearman was used to analyze the correlation between AT1-AA level and follicle development indexes, and multiple linear regression was used to adjust for interfering factors to analyze the correlation between AT1-AA level and embryonic laboratory indicators. Results:Body mass index [BMI, (25.29±3.25) kg/m 2], antral follicle count [AFC, 24.00 (17.00, 24.00)], basal serum teststerone [0.50 (0.36, 0.79) μg/L] and anti-Müllerian hormone [AMH, 4.92 (2.57, 8.28) μg/L] in PCOS group were significantly higher than those in tubal factor group [(23.01±2.92) kg/m 2, P<0.001; 15.00 (10.25, 19.00), P<0.001; 0.38 (0.20, 0.59) μg/L, P=0.019; 3.06 (2.19, 4.89) μg/L, P=0.006]. Moreover, the AT1-AA P/N value in follicular fluid in PCOS group [4.24 (2.07, 8.89)] was significantly higher than that in tubal factor group [2.96 (1.86, 4.84), P=0.027]. In addition, the age of menarche in PCOS group [13.00 (12.00, 14.00) years] was earlier than that in tubal factor group [14.00 (13.00, 15.00) years, P=0.007]. Prolactin level [12.09 (9.65, 16.64) μg/L] was lower than that of tubal factor group [16.27 (12.86, 20.94) μg/L, P=0.002], and there were no statistical differences in other indexes between the two groups (all P>0.05). Correlation analysis showed that the level of AT1-AA in follicular fluid of PCOS patients was negatively correlated with oocyte retrieval rate ( r=-0.159, P=0.019). The results of multiple linear regression analysis showed that AT1-AA level in follicular fluid was negatively correlated with oocyte retrieval rate ( β=-0.598, P=0.028) and normal fertilization rate ( β=-0.527, P=0.022) after adjusting for confounding factors. Conclusion:The level of AT1-AA in follicular fluid in PCOS patients is significantly higher than that in patients with tubal infertility, and is negatively correlated with oocyte retrieval rate and normal fertilization rate.
