Effects of nanosilicate functionalized polycaprolactone membrane on bone mesenchymal stem cells-induced bone repairing
10.13591/j.cnki.kqyx.2025.08.002
- VernacularTitle:纳米硅酸镁锂功能化聚己内酯膜对骨髓间充质干细胞介导骨再生修复的作用研究
- Author:
Long XIAO
1
;
Weiqiang HU
1
;
Xuxin LIN
1
;
Mengjiao HE
1
;
Kai LUO
1
;
Xiongcheng XU
1
Author Information
1. 福建省口腔疾病研究重点实验室,福建省口腔生物材料工程技术研究中心,福建医科大学口腔组织工程研究中心,福建医科大学附属口腔医院,福建 福州(350002)
- Publication Type:Journal Article
- Keywords:
barrier membrane;
polycaprolactone;
nanosilicate;
bone marrow mesenchymal stem cells;
bone regeneration
- From:
STOMATOLOGY
2025;45(8):567-575
- CountryChina
- Language:Chinese
-
Abstract:
Objective To fabricate nanosilicate functionalized polycaprolactone(PCL/LAP)electrospun membrane and evaluate its role in bone marrow mesenchymal stem cells(BMSCs)-induced bone repairing.Methods The PCL/LAP electrospun membranes were fabricated via electrospinning technology and co-cultured with rat BMSCs.The cytocompatibility of the membranes was evaluated through cytoskeleton staining,live/dead cell staining and CCK-8 assay.The migration capacity of BMSCs was assessed using scratch assay,Transwell migration experiments and expression of migration-related genes(Pdgf and Tgfβ)was evaluated by qRT-PCR.The os-teogenic differentiation and pro-angiogenesis potential were determined by alkaline phosphatase(ALP)staining,alizarin red staining,expression levels of osteogenesis-related genes(Alp,Col1a1,Runx2,Bglap and Bmp2)and angiogenesis-related genes(Angpt1,Fgf2 and Vegfa)along with RUNX2 protein expression.PCL and PCL/LAP electrospun membranes conditioned medium was subsequently used to stimulate vascular endothelial cells(EAhy926).The expression of angiogenesis-associated genes(KDR,ENOS and HIF1A)was quantified by qRT-PCR.Results BMSCs adhered well to the surface of the PCL/LAP membranes,with no significant impact on cell viability(P＞0.05).PCL/LAP membranes not only promoted the proliferation(P＜0.05),migration(P＜0.05),but also enhanced ALP activity and mineralized nodule formation(P＜0.05),increased osteogenic differentiation gene and protein expression(P＜0.05)of BMSCs.Moreover,PCL/LAP promoted the expression of angiogenic genes of BMSCs(P＜0.05),to indirectly regulate angiogenesis-related gene expression in vascular endothelial cells(P＜0.05).Conclusion PCL/LAP electrospun membranes exhibit excellent biocompatibility and can promote proliferation,migration,osteogenic differentiation and BMSC-mediated angiogenic differentiation,showing great potential for bone defect repairing as barrier membrane.
