Influence of morphological evaluation parameters of blastocysts on chromosomal karyotype abnormalities of chorionic villi in missed abortion after IVF/ICSI treatment
10.3760/cma.j.cn101441-20231122-00329
- VernacularTitle:囊胚形态学评价参数对IVF/ICSI助孕后稽留流产绒毛染色体核型异常的影响
- Author:
Mengna LI
1
;
Wei ZHENG
1
;
Chen YANG
1
;
Bingnan REN
1
;
Erfeng YUAN
1
;
Songzhan GAO
1
;
Ruowen ZU
1
;
Yihui KUANG
1
;
Jing WU
1
;
Mengyi CAO
1
;
Jingyi HAN
1
;
Peixin LI
1
;
Ran SHEN
1
;
Yichun GUAN
1
Author Information
1. 郑州大学第三附属医院生殖医学中心，郑州 450052
- Publication Type:Journal Article
- Keywords:
Single blastocyst;
Embryo morphological parameters;
Inner cell mass;
Trophectoderm;
Missed abortion;
Chromosome of chorionic villi
- From:
Chinese Journal of Reproduction and Contraception
2024;44(6):628-634
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Analyzing the influence of morphological evaluation parameters of blastocysts, including days of blastocyst development [day 5 (D5) and day 6 (D6)], degree of blastocyst expansion (4, 5, 6), inner cell mass and trophectoderm grade, on the occurrence of chromosomal karyotype abnormalities of chorionic villi in missed abortion after in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment and fresh/frozen-thawed single blastocyst transfer. Methods:The clinical data of patients with missed abortion after IVF/ICSI treatment and fresh/frozen-thawed single blastocyst transfer from February 2015 to February 2023 in the Reproductive Center of the Third Affiliated Hospital of Zhengzhou University were included. Using a case-control study, the data were divided into two groups according to the detection results of chromosomal copy number variations (CNVs) in chorionic villi of missed abortion abnormal karyotype group ( n=139) and normal karyotype group ( n=82). The baseline data between the two groups were compared. Univariate logistic regression was used to investigate the effect of blastocyst morphological rating parameters on the occurrence of chromosomal karyotype abnormalities of chorionic villi in aborted tissues, and multivariate logistic regression was also used to adjust confounding factors. Results:Male age [(34.12±6.49) years], sperm morphology rate [5.00 (4.00,6.00)%] and female age [33.00 (30.00, 37.00) years] in abnormal karyotype group were higher than those in the normal karyotype group [(32.38±4.69) years, 4.00 (2.00,5.00)% and 31.50 (29.00,34.00) years], and the differences were statistically significant ( P=0.022, P=0.020, P=0.009). Univariate and multivariate logistic regression analyses showed that days of blastocyst development, degree of blastocyst expansion, inner cell mass and trophectoderm grade did not increase the risk of chromosomal karyotype abnormalities of chorionic villi (all P>0.05). Conclusion:There is no significant correlation between blastocyst morphological evaluation parameters and chromosomal karyotype abnormalities in chorionic villi of missed abortion after fresh/frozen-thawed single blastocyst transfer with IVF/ICSI treatment.
