Effect of Mito TEMPO on liquid preservation of boar semen
10.16303/j.cnki.1005-4545.2024.12.23
- VernacularTitle:Mito TEMPO对猪精液液态保存的影响
- Author:
Xiaoqi BIAN
1
;
Shunli YUE
1
;
Xiaogang WENG
1
;
Xue LIU
1
;
Chang DING
1
;
Jiaxuan LI
1
;
Jiabo ZHOU
1
Author Information
1. 东北农业大学 生命科学学院 黑龙江省动物细胞与遗传工程重点实验室,黑龙江哈尔滨 150030
- Publication Type:Journal Article
- Keywords:
boar semen;
liquid preservation;
Mito TEMPO;
mitochondrial membrane potential
- From:
Chinese Journal of Veterinary Science
2024;44(12):2661-2666
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to investigate the effect of Mito TEMPO on the preservation of porcine semen in liquid form.The preservation solution was supplemented with 0,0.5,5.0,50.0,and 500.0 μmol/L of Mito TEMPO,and the diluted boar semen was stored at 17 ℃ for 1 to 5 days.After preservation,we measured sperm motility,acrosome integrity,plasma membrane integrity,reactive oxygen species(ROS)content,total antioxidant capacity,and mitochondrial membrane potential.The fertilization ability and embryonic development potential of sperm preserved for 5 days were assessed using an in vitro fertilization test.The results indicated that the addition of 5.0,50.0 μmol/L of Mito TEMPO to the preservation solution significantly reduced ROS content and improved sperm motility,plasma membrane integrity,acrosome integrity,mitochondrial membrane potential,and antioxidant capacity.Furthermore,it enhanced the fertilization and embryonic devel-opment potential of the preserved sperm.Therefore,Mito TEMPO is beneficial for the preservation of porcine semen in liquid form and may serve as a foundation for the development of liquid semen preservation solutions.
