Polymorphism analysis of PAPSS2 gene and the association with milk quality traits in Chinese Holstein dairy cows
10.16303/j.cnki.1005-4545.2024.12.21
- VernacularTitle:中国荷斯坦奶牛PAPSS2基因遗传多态性及与乳品质性状的相关性分析
- Author:
Panpan XU
1
;
Quanheng GUO
1
;
Qiwen LU
1
;
Haibin YU
1
;
Ziwei LIN
1
;
Zhihui ZHAO
1
;
Ping JIANG
1
Author Information
1. 广东海洋大学滨海农业学院,广东湛江 524088
- Publication Type:Journal Article
- Keywords:
Chinese Holstein dairy cows;
PAPSS2 gene;
single nucleotide polymorphism;
milk pro-duction traits
- From:
Chinese Journal of Veterinary Science
2024;44(12):2650-2654,2660
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the relationship between single nucleotide polymorphisms(SNPs)in the 3'-phosphoadenosine 5'-phosphosulfate synthase 2(PAPSS2)gene and milk quality traits in Chinese Holstein cows.A total of 141 Chinese Holstein cows were sampled,and their DNA was extracted to detect polymorphic sites in the PAPSS2 gene using direct sequencing technology.The genotypes at different SNP sites were then correlated with milk quality traits.The results i-dentified three SNP sites in the second intron of the PAPSS2 gene:I2-3686 A＞G,I2-3854 G＞C,and I2-3917 G＞A,each exhibiting three genotypes.Specifically,at the first SNP site,individuals with the AG genotype demonstrated significantly higher milk fat and solids content compared to those with the GG genotype(P＜0.05).At the second SNP site,individuals with the GG genotype exhibited significantly higher urinary nitrogen content than those with the GC and CC genotypes(P＜0.05).No significant differences were observed in milk quality traits associated with the third SNP site.These findings suggest that SNP sites in the PAPSS2 gene can serve as molecular mark-ers influencing milk quality traits in Chinese Holstein cows,providing potential applications for marker-assisted selection in dairy cattle.
