Analysis of LRP5 gene polymorphisms and the correlation with milk quality traits in Chinese Holstein dairy cows
10.16303/j.cnki.1005-4545.2024.12.20
- VernacularTitle:中国荷斯坦奶牛LRP5基因多态性及其与乳品质性状的相关性分析
- Author:
Quanheng GUO
1
;
Panpan XU
1
;
Qiwen LU
1
;
Haibin YU
1
;
Ziwei LIN
1
;
Junyu WANG
1
;
Zhihui ZHAO
1
;
Ping JIANG
1
Author Information
1. 广东海洋大学 滨海农业学院动物遗传与分子育种实验室,广东湛江 524088
- Publication Type:Journal Article
- Keywords:
Chinese Holstein dairy cows;
LRP5 gene;
single nucleotide polymorphism;
milk produc-tion traits
- From:
Chinese Journal of Veterinary Science
2024;44(12):2644-2649
- CountryChina
- Language:Chinese
-
Abstract:
In order to reveal the association between single nucleotide-based polymorphism(low density lipoprotein receptor-related protein 5,LRP5),DNA of 98 Chinese Holstein cows were ex-tracted and the LRP 5 gene polymorphism locus was detected by direct sequencing,and related dif-ferent genotypes and milk quality traits at the SNP loci was analyzed.The results showed that three SNPs were found on the fourth intron of the LRP 5 gene,I4-90 C＞T,I4-229 C＞A and I4-570 A＞G.Among them,I4-90 C＞T had TT,CC and TC genotypes,and TC genotype was signifi-cantly higher than TT and CC genotypes(P＜0.05).However,mutations at this locus were not significantly associated with milk yield and fat content(P＞0.05);genotypes CC and C for I4-229 C＞A;genotypes AA,GG and GA for I4-570 A＞G.The two mutation sites showed no significant correlation with milk quality traits in Chinese Holstein dairy cows(P＞0.05).The above results indicate that the I4-90 C＞T site of LRP 5 gene can be used for marker-assisted selection as molec-ular markers affecting milk quality traits in Chinese Holstein cows.
