Protective effect of polysacchcride of Atractylodes macrocephala koidz.on lipopo-lysaccharide induced acute kidney injury in juvenile laying hen
10.16303/j.cnki.1005-4545.2024.12.19
- VernacularTitle:白术多糖对脂多糖诱导蛋雏鸡急性肾损伤的保护作用
- Author:
Yujia WU
1
;
Siyuan LIU
;
Xu LIU
;
Heping BAI
;
Qian LI
;
Xiaodan WANG
Author Information
1. 河北农业大学 中兽医学院,河北 保定 071000
- Publication Type:Journal Article
- Keywords:
polysaccharide of Atractylodes macrocephala koidz.;
acute kidney injury;
chicken;
in-flammation;
apoptosis
- From:
Chinese Journal of Veterinary Science
2024;44(12):2635-2643
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of the polysaccharide derived from Atractylodes macrocephala koidz.(PAMK)on lipopolysaccharide(LPS)-induced acute kidney injury in juvenile laying hens.A total of 100 one-day-old male Hy-Line brown laying hens were randomly divided into five groups:a control group(CON group),an LPS treatment group(LPS group),a PAMK low-dose group(LPS+PAMK 250 mg/L,group A),a PAMK medium-dose group(LPS+PAMK 500 mg/L,group B),and a PAMK high-dose group(LPS+PAMK 1 000 mg/L,group C).The PAMK-treated groups were fed with corresponding doses of PAMK mixed with water daily from 1-20 days of age.At the age of 14,16,18 and 20 days,the chickens in the LPS group and PAMK-treated groups were intraperitoneally injected with LPS,while the chickens in the blank control group were injec-ted with the same volume of normal saline.After feeding for 21 days,the kidneys were collected,and pathological changes in kidney tissue sections were observed using hematoxylin and eosin(HE)staining.Kidney weight and chick weight were measured to calculate the kidney coefficient.The levels of BUN,CRE,MDA,SOD,GSH-Px and T-AOC in kidney tissues were measured.The contents of TNF-α and IL-1β were determined by ELISA.The mRNA expression of inflammatory factor genes(IL-1β,TNF-α and NF-κB)and apoptosis-related factors(p53,Bcl-2,Bax and Caspase-3)in the kidneys was detected using quantitative real-time PCR.The results indicated that PAMK alleviated LPS-induced renal tissue injury.Compared to the LPS group,PAMK significantly re-duced the kidney coefficient and BUN concentration in the kidneys(P＜0.05).Additionally,the levels of TNF-α and IL-1β,as well as the mRNA expression of TNF-α,IL-1β,and NF-κB in the PAMK group,were decreased compared to the LPS group.Furthermore,T-AOC,SOD and GSH-Px activities in the PAMK group were significantly increased(P＜0.05),while MDA content was significantly decreased(P＜0.01).Compared with the LPS group,the mRNA expression of Bax,Caspase-3 and p53 in the PAMK low-dose group and the PAMK medium-dose group decreased,while the mRNA expression of Bcl-2 increased.In conclusion,PAMK can inhibit the inflammatory response and oxidative stress response caused by LPS,and alleviate the apoptosis of kidney cells,and the treatment effect of PAMK in the middle dose group is the best,which provides a theoreti-cal basis for PAMK treatment of acute kidney injury in chickens.
