Rehmannia extract enhances ovarian functions of chicken via regulating PI3K/AKT/mTOR signaling pathway
10.16303/j.cnki.1005-4545.2024.12.17
- VernacularTitle:熟地黄提取物调控PI3K/AKT/mTOR信号通路增强鸡卵巢功能
- Author:
Yating YANG
1
;
Xiaotong WANG
;
Zhaomei LIU
;
Yangyong DONG
;
Yuxuan ZHANG
;
Ran WANG
;
Chiyu PAN
;
Xiaoyan QIU
;
Xiong XIAO
Author Information
1. 西南大学动物医学院,重庆荣昌 400715
- Publication Type:Journal Article
- Keywords:
Rehmannia extract;
black skin and red crest chicken;
ovary;
PI3K;
AKT;
mTOR
- From:
Chinese Journal of Veterinary Science
2024;44(12):2619-2625,2680
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the mechanism by which Rehmannia extract enhances ovarian follicle development,reduces ovarian aging,and improves the production performance of chickens,35-week-old black skin and red crest chickens were randomly divided into a blank control group and groups supplemented with 0.1％,0.2％and 0.4％Rehmannia extract for feeding experiments.Changes of histological structure and molecular regulation for improving the reproductive perform-ance of black skin and red crest chicken were explored here by detecting the reproductive hormones and the corresponding receptors in ovary and quantifying the cell proliferation and apoptotic lesion.Based on the PI3K/AKT/mTOR and AMPK signaling pathways,mechanisms of Rehmannia ex-tract on promoting ovarian follicle development and alleviating ovarian aging were further studied to enrich the reproductive regulation theory of native breed chickens and improve their reproduc-tive performance.The results indicated that 0.4％Rehmannia extract could improve the levels of LH,E2 and P4 in serum,elevate the relative expression levels of FSHR,ERβ and PCNA mRNA in ovarian tissues,increase the content of PCNA protein,and decrease the number of apoptosis cells and the level of apoptosis protein Cleaved-Caspase-3.By enhancing the secretion of norepinephrine and activating the a2A-AR and the downstream PI3K/AKT/mTOR signaling pathway in ovary,the Rehmannia extract could effectively relieve the decline of ovarian function and maintain stable and efficient ovarian functions,which was independent of AMPK signaling pathway.Therefore,0.4％Rehmannia extract is conducive to enhancing the reproductive function via regulating PI3K/AKT/mTOR signaling pathway.
