Effects of activating and suppressing GPR120 gene on milk lipidmetabolism and inflammatory response of dairy cattle MAC-T cells
10.16303/j.cnki.1005-4545.2024.12.22
- VernacularTitle:激活和抑制GPR120基因对奶牛MAC-T细胞乳脂代谢及炎性反应的影响
- Author:
Lin WAN
1
;
Peiyao ZHOU
;
Quanzhou MU
;
Xinli LI
;
Siqi WANG
;
Yang LI
;
Xingli HE
;
Zhaoyuan WANG
;
Zi WANG
;
Ziqiang GAO
;
Zhihui ZHAO
;
Binglei SHEN
Author Information
1. 黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319
- Publication Type:Journal Article
- Keywords:
GPR120 gene;
MAC-T cells;
milk fat synthesis;
inflammatory factors
- From:
Chinese Journal of Veterinary Science
2024;44(12):2655-2660
- CountryChina
- Language:Chinese
-
Abstract:
As a receptor protein,GPR120 is activated by long-chain fatty acids(such as omega-3 fat-ty acids,alpha-linolenic acid,eicosapentaenoic acid,and docosahexaenoic acid.It plays an important regulatory role in gastrointestinal peptide release,inflammation,lipogenesis,glucose tolerance,and insulin sensitivity.In order to study the synergistic regulation of the GPR120 gene on milk fat me-tabolism and its anti-inflammatory effects in dairy cow MAC-T cells,the GPR120 activator TUG-891 and the inhibitor AH7614 were used to treat both dairy cow MAC-T cells and LTA-induced inflammatory dairy cow MAC-T cells.This treatment aimed to detect the expression of key genes involved in milk fat synthesis and inflammatory factors.The results showed that the GPR120 gene activator significantly increased the relative expression levels of cholesterol regulatory element binding protein(SREBP1)and fatty acid synthase(FASN),key genes for milk fat synthesis.Addi-tionally,the expression levels of the inflammatory factor interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were reduced in the inflammatory dairy cow MAC-T cell model,which prelimi-natively reveals that GPR120 co-regulates milk fat and mammary inflammation in dairy cows,thereby laying a foundation for subsequent molecular mechanism research.
