Effect of exogenous luteinizing hormone on vitrification granulosa cells and its mechanism
- VernacularTitle:外源性黄体生成素对玻璃化冻存颗粒细胞的影响及机制研究
- Author:
Jie CHEN
1
;
Shang GAO
;
Shao-jie ZHANG
;
Xi YANG
;
Kun LI
;
Jun SHI
;
Xing WANG
Author Information
- Publication Type:Journal Article
- Keywords: granulosa cells; vitrification; luteinizing hormone; Foxl2; PI3K/AKT/mTOR pathway
- From: Journal of Regional Anatomy and Operative Surgery 2025;34(1):1-5
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of exogenous luteinizing hormone (LH) on vitrification granulosa cells and its mecha-nisms. Methods The granulosa cells of 4-week-old preadolescent mice were selected and divided into the fresh group (without vitrification procedure),the vitrification group (treated according to vitrification procedure) and the LH group (treated with 0.3 IU/L of LH intervention on the basis of the vitrification group). Granulocyte count was performed,and Realtime PCR and Western blot were used to detect the expression changes of Foxl2,PI3K,AKT and mTOR mRNAs and proteins in each group. Results Compared with the vitrification group,the total number of granulosa cells in the LH group was significantly increased (P<0.05),and the expression of Foxl2,PI3K,AKT and mTOR mRNAs and proteins were elevated (P<0.05). Conclusion The intevention of exogenous LH is beneficial for the survival of vitrifi-cation granulosa cells,and the mechanism may be related to the activation of PI3K/AKT/mTOR pathway.
