Expression levels and molecular mechanisms of LncHCG11 and miR-214-5p in pancreatic cancer
10.13431/j.cnki.immunol.j.20250061
- VernacularTitle:LncHCG11、miR-214-5p在胰腺癌中的表达水平及分子机制
- Author:
Wenbin PENG
1
;
Yuanyuan YIN
;
Yuling TANG
;
Xiangyang TANG
Author Information
1. 422000 邵阳,邵阳学院附属第一医院消化内科
- Publication Type:Journal Article
- Keywords:
LncHCG11;
miR-214-5p;
Sirtuin 2;
Pancreatic tumor;
Panc1 cell line;
Cell proliferation;
Real-time quantitative PCR
- From:
Immunological Journal
2025;41(6):417-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective Objective To investigate the expression levels of long non-coding RNA HCG11(LncHCG11)and miR-214-5p in pancreatic cancer tissues and their potential molecular regulatory mechanisms.Methods Ten patients with pancreatic cancer admitted between January 2022 and January 2025 were included,and post-operative cancer tissue and paired adjacent tissue samples were collected.Real-time quantitative PCR technology was used to detect the transcription levels of LncHCG11 and miR-214-5p,and the expression level of sirtuin 2(SIRT2)protein was measured by Western blot.The relationship of miR-214-5p with LncHCG11 and SIRT2 was predicted through a biological database.Results Compared with the adjacent non-cancerous tissues,the expression of LncHCG11 and SIRT2 was significantly reduced in pancreatic cancer tissues,while the level of miR-214-5p was significantly increased(P<0.01).In vitro experiments showed that overexpression of LncHCG11 in the Panc1 cell line could upregulate the expression of SIRT2 protein and simultaneously inhibit the proliferative activity of Panc1 cells(P<0.05).Both miR-214-5p and LncHCG11,SIRT2 had binding sites.Conclusion LncHCG11 may act as a competing endogenous RNA to sponge miR-214-5p,releasing its transcriptional inhibition on SIRT2,thereby inhibiting the progression of pancreatic cancer.
